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CSK Antibody (CSK-04) - BSA Free

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Immunocytochemistry/ Immunofluorescence: CSK Antibody (CSK-04) [NB500-344] - Staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
CSK-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CSK Antibody (CSK-04) - BSA Free Summary

Immunogen
Bacterially expressed recombinant fragment of human Csk corresponding to amino acids 330-450.
Localization
Nuclear
Specificity
The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSK
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1:200-1:300
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CSK Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CSK Antibody (CSK-04) - BSA Free

  • CSK
  • C-Src kinase
  • c-src tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • MGC117393
  • Protein-tyrosine kinase CYL
  • tyrosine-protein kinase CSK

Background

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CSK Antibody (NB500-344) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for CSK Antibody (NB500-344)

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Blogs on CSK

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol CSK
Entrez