Home » Common gamma Chain/IL-2 R gamma » Common gamma Chain/IL-2 R gamma Antibodies » Common gamma Chain/IL-2 R gamma Antibody (3E12) [Janelia Fluor® 549]
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Common gamma Chain/IL-2 R gamma Antibody (3E12) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IP, B/N
Clone
3E12
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-12038AF350
Alexa Fluor 405
NBP3-12038AF405
Alexa Fluor 488
NBP3-12038AF488
Alexa Fluor 532
NBP3-12038AF532
Alexa Fluor 594
NBP3-12038AF594
Alexa Fluor 647
NBP3-12038AF647
Alexa Fluor 700
NBP3-12038AF700
Alexa Fluor 750
NBP3-12038AF750
Allophycocyanin
NBP3-12038APC
Biotin
NBP3-12038B
DyLight 350
NBP3-12038UV
DyLight 405
NBP3-12038V
DyLight 488
NBP3-12038G
DyLight 550
NBP3-12038R
DyLight 594
NBP3-12038DL594
DyLight 650
NBP3-12038C
DyLight 680
NBP3-12038FR
DyLight 755
NBP3-12038IR
FITC
NBP3-12038F
HRP
NBP3-12038H
Janelia Fluor 646
NBP3-12038JF646
PE
NBP3-12038PE
PerCP
NBP3-12038PCP
Unconjugated
NBP3-12038-0.2mg
mFluor Violet 450 SE
NBP3-12038MFV450
mFluor Violet 500 SE
NBP3-12038MFV500
mFluor Violet 610 SE
NBP3-12038MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-12038-0.2mg
Azide and BSA Free
NBP3-12038-0.2mg
Azide and BSA Free
NBP3-12038-0.025mg
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Common gamma Chain/IL-2 R gamma Antibody (3E12) [Janelia Fluor® 549] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).
Specificity
This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognises an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
IL2RG
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody (3E12) [Janelia Fluor® 549]

  • CD132 antigen
  • CD132
  • CIDX
  • combined immunodeficiency, X-linked
  • common cytokine receptor gamma chain
  • Common gamma Chain
  • common gamma-chain
  • cytokine receptor common subunit gamma
  • gamma(c)
  • IL-2 R gamma
  • IL-2 receptor subunit gamma
  • IL2R gamma
  • IL-2R subunit gamma
  • IL2RG
  • IL-2RG
  • IMD4
  • interleukin 2 receptor, gamma
  • Interleukin-2 receptor subunit gamma
  • P64
  • SCIDX
  • SCIDX1
  • severe combined immunodeficiency

Background

The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors(IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked genecause X-linked severe combined immunodeficiency (XSCID). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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mFluor Violet Conjugated Antibodies

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As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
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Bioinformatics

Gene Symbol IL2RG