![Western Blot: ChABC Antibody (1E10) - Azide and BSA Free [NBP2-80665] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ChABC-Antibody-1E10-Azide-and-BSA-Free-Western-Blot-NBP2-80665-img0001.jpg "Western Blot: ChABC Antibody (1E10) - Azide and BSA Free [NBP2-80665] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information. Image from the standard format of this antibody.")
| Reactivity | Rt, All-NASpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1E10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Chondroitinase ABC |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This ChABC (1E10) antibody is useful for ELISA and Western blot, where a band can be seen at approximately 80 kDa. It can be used in Sandwich ELISA as the detection antibody with catalog # NBP1-96142, ChABC (6A12) as the capture antibody. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Western blot data: 125 ng Chondroitinase ABC was mixed with 100 ug of fetal bovine serum (as a background control). The proteins were mixed with 100 ul of non-reducing sample buffer and applied to a single 6 cm well set in a SDS-PAGE minigel (approx 20 ng/well [0.5 cm]). The gel bands were electroblotted to a nitrocellulose sheet. Immunolabeling for individual antibodies was performed on the sheet placed in a slot blot devise. Purified NBP1-96141 (ChABC/1E10) and NBP1-96142 (ChABC/6A12) were applied at 5 ug/ml IgG. |
| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")