![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0007.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).")
| Reactivity | Hu, Rt, Po, Bv, Ch, Eq, Gp, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC (-) |
| Clone | PC3/188A |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54405) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain (Uniprot: P07766 ) |
| Localization | Cell surface and Cytoplasmic |
| Marker | T-Cell Marker |
| Specificity | Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD3E |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Optimal dilution for a specific application should be determined. Western Blot: 1-2ug/ml for 60 minutes at RT. |
|
| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD3 epsilon Antibody (NBP2-53386)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Immunohistochemistry-Paraffin-NBP2-53386-img0001.jpg "Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).")
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0005.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).")
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0006.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (PC3/188A).")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")