| Reactivity | MuSpecies Glossary |
| Applications | B/N |
| Clone | 123616 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # P10148 |
| Specificity | Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human
(rh) CCL1, 2, 3,
4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,
rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12,
17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | Ccl2 |
| Purity | Protein A or G purified from hybridoma culture supernatant |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Purity | Protein A or G purified from hybridoma culture supernatant |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
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