![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) [NBP2-32919] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (SPM166).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-SPM166-Immunohistochemistry-Paraffin-NBP2-32919-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) [NBP2-32919] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (SPM166).")
| Reactivity | All-NASpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | SPM166 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34744) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Bromodeoxyuridine/BrdU conjugated to KLH |
| Localization | Nuclear |
| Marker | Proliferation Marker |
| Specificity | It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Use Ab at 1ug/ml for 30 minute at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 307 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
| Publications using NBP2-32919 | Applications | Species |
|---|---|---|
| Pushchina EV, Stukaneva ME, Varaksin AA et al. Damage-related changes in the cerebellum of juvenile Oncorhynchus masou: reactivation of neurogenic niches and astrocytic response bioRxiv (IHC-P, Fish) Details: (O. masou) |
IHC-P | Fish |
| Pushchina EV, Zharikova EI, Varaksin AA et al. Proliferation, Adult Neuronal Stem Cells and Cells Migration in Pallium during Constitutive Neurogenesis and after Traumatic Injury of Telencephalon of Juvenile Masu Salmon, Oncorhynchus masou Brain Sci 2020-04-08 [PMID: 32276413] (IHC-P, IF/IHC, Fish) | IHC-P, IF/IHC | Fish |
| Pushchina EV, Kapustyanov IA, Varaksin AA Neural Stem Cells/Neuronal Precursor Cells and Postmitotic Neuroblasts in Constitutive Neurogenesis and After ,Traumatic Injury to the Mesencephalic Tegmentum of Juvenile Chum Salmon, Oncorhynchus keta Brain Sci 2020-01-25 [PMID: 31991815] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Bromodeoxyuridine/BrdU Antibody (NBP2-32919)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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BrDU (Bromodeoxyuridine) The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2). Because BrDU is only incorporated in... Read full blog post. |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")