![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (MoBu-1).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-MoBu-1-Immunohistochemistry-Paraffin-NB500-439-img0003.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (MoBu-1).")
| Reactivity | All-NASpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | MoBu-1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-68041) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Bromodeoxyuridine/BrdU conjugated to hemocyanine (isolated from Helix pomatia) |
| Localization | Nuclear |
| Marker | Proliferation Marker |
| Specificity | It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C. Optimal dilution for a specific application should be determined. |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Bromodeoxyuridine/BrdU Antibody (NB500-439)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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BrDU (Bromodeoxyuridine) The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2). Because BrDU is only incorporated in... Read full blog post. |
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![Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Staining of bromodeoxyuridine/BrdU-labeled cells (chick embryo; paraffin-embedded sections)](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-MoBu-1-Immunohistochemistry-NB500-439-img0002.jpg "Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Staining of bromodeoxyuridine/BrdU-labeled cells (chick embryo; paraffin-embedded sections)")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")