![Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Western blot of Bcl-2 in human A) HeLa B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0003.jpg "Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Western blot of Bcl-2 in human A) HeLa B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8)")
| Reactivity | Hu, Po, Pm, Mu(-), Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 8C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29630). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 ) |
| Localization | Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. |
| Marker | Apoptosis & Follicular Lymphoma Marker |
| Specificity | This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCL2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | BCL2 |
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Formalin-fixed paraffin-embedded human tonsil stained with Bcl-2 Monoclonal Antibody (8C8) Note cell membrane and cytoplasmic staining](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33313-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Formalin-fixed paraffin-embedded human tonsil stained with Bcl-2 Monoclonal Antibody (8C8) Note cell membrane and cytoplasmic staining")
![Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Analysis of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0001.jpg "Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Analysis of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")