![Immunohistochemistry-Paraffin: AMPD3 Antibody (AMPD3/901) - Azide and BSA Free [NBP2-47691] - Human Tonsil stained with AMPD3 Monoclonal Antibody (AMPD3/901)](http://images.novusbio.com/fullsize/AMPD3-Antibody-AMPD3-901-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47691-img0002.jpg "Immunohistochemistry-Paraffin: AMPD3 Antibody (AMPD3/901) - Azide and BSA Free [NBP2-47691] - Human Tonsil stained with AMPD3 Monoclonal Antibody (AMPD3/901)")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | AMPD3/901 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44441). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant full-length human AMPD3 protein (Uniprot: Q01432) |
| Localization | Cell Surface |
| Marker | Erythroid Marker |
| Specificity | It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This monoclonal antibody shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This monoclonal antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AMPD3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 90 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | AMPD3 |
![Immunohistochemistry-Paraffin: AMPD3 Antibody (AMPD3/901) - Azide and BSA Free [NBP2-47691] - Human Placenta stained with AMPD3 Monoclonal Antibody (AMPD3/901)](http://images.novusbio.com/fullsize/AMPD3-Antibody-AMPD3-901-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47691-img0001.jpg "Immunohistochemistry-Paraffin: AMPD3 Antibody (AMPD3/901) - Azide and BSA Free [NBP2-47691] - Human Placenta stained with AMPD3 Monoclonal Antibody (AMPD3/901)")
