![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).](http://images.novusbio.com/fullsize/AKR1B10-Antibody-Western-Blot-NB100-93370-img0003.jpg "Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QSSHLEDYPFDAE corresponding to C-Terminus according to NP_064695.2. |
| Epitope | C-QSSHLEDYPFDAE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | AKR1B10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 35 kDa band observed in lysates of HEK293, A549 and HepG2 (calculated MW of 36.0 kDa band according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)Find related products by research area.
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| Gene Symbol | AKR1B10 |
| Entrez |
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| Uniprot |
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![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AKR1B10-Antibody-Western-Blot-NB100-93370-img0002.jpg "Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
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