![Knockout Validated: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and AHSA1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with antiAHSA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with antiHSP90AB1antibody as a loading control.(1:500)](http://images.novusbio.com/fullsize/AHSA1-Antibody-OTI1D2-Western-Blot-NBP2-46503-img0009.jpg "Knockout Validated: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and AHSA1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with antiAHSA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with antiHSP90AB1antibody as a loading control.(1:500)")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, KO |
| Clone | OTI1D2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-203 of human AHSA1 (NP_036243) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AHSA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | AHSA1 |
![Western Blot: AHSA1 Antibody (OTI1D2) [NBP2-46503] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiAHSA1.(1:2000)](http://images.novusbio.com/fullsize/AHSA1-Antibody-OTI1D2-Western-Blot-NBP2-46503-img0011.jpg "Western Blot: AHSA1 Antibody (OTI1D2) [NBP2-46503] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiAHSA1.(1:2000)")
![Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiAHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/AHSA1-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-46503-img0006.jpg "Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiAHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
![Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)](http://images.novusbio.com/fullsize/AHSA1-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-46503-img0002.jpg "Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)")
![Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/AHSA1-Antibody-OTI1D2-Immunohistochemistry-Paraffin-NBP2-46503-img0004.jpg "Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")