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![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0001.jpg "PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0002.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0008.jpg)
![PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize/PiColorlock-Gold-Colorimetric-Assay-Kit-303-0030-img0007.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.](http://images.novusbio.com/fullsize/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0003.jpg "PiColorLock Gold Colorimetric Assay Kit [303-0125] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the addition of an acidified dye reagent (orange), which turns green or yellow in the presence or absence of inorganic phosphate.")
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate forms and settles. In plate assays, the data become extremely erratic when clumps of material form in the light path. PiColorlock complexes are stable for many hours, which allows multiple assay plates or cuvettes to be conveniently set up as a batch and read later using plate stackers or other automated equipment.](http://images.novusbio.com/fullsize/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0004.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.](http://images.novusbio.com/fullsize/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0005.jpg)
![PiColorLock Gold Colorimetric Assay Kit [303-0125] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, at least at low levels of inorganic phosphate. Competitors' products are linear over a much narrower range of concentrations. PiColorlock ALS reagent has now been superseded by PiColorlock Gold, which gives higher OD values.](http://images.novusbio.com/fullsize/PiColorLock-Gold-Colorimetric-Assay-Kit-303-0125-img0006.jpg)
![pCL-Eco Retrovirus Packaging Vector [NBP2-29540]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29540-img0001.jpg "pCL-Eco Retrovirus Packaging Vector [NBP2-29540]")
![pCLXSN Retrovirus Expression Vector [NBP2-29500] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).](http://images.novusbio.com/fullsize/pCLXSN-Retrovirus-Expression-Vector-NBP2-29500-img0001.jpg "pCLXSN Retrovirus Expression Vector [NBP2-29500] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).")
![pCLNDX Retrovirus Expression Vector [NBP2-29539]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29539-img0001.jpg "pCLNDX Retrovirus Expression Vector [NBP2-29539]")
![pCLNDX Retrovirus Expression Vector [NBP2-29539]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29539-img0002.jpg)
![pCLNDX Retrovirus Expression Vector [NBP2-29539]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29539-img0003.jpg)
![pCLNDX Retrovirus Expression Vector [NBP2-29539]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29539-img0004.jpg)
![pCLNDX Retrovirus Expression Vector [NBP2-29539]](http://images.novusbio.com/fullsize/pCLNDX-Retrovirus-Expression-Vector-NBP2-29539-img0005.jpg)
![Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07085-img0001.jpg "Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-NBA1-07085-img0002.jpg)
![Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/N-OLLAS-Vector-Western-Blot-NBA1-07083-img0002.jpg "Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![N OLLAS Vector [NBA1-07083] - Vector map of pCMV-N OLLAS](http://images.novusbio.com/fullsize/N-OLLAS-Vector-NBA1-07083-img0003.jpg)