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![Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/VPS26-Antibody-Western-Blot-NBP2-75922-img0001.jpg "Western Blot: VPS26 Antibody [NBP2-75922] - Total protein from A431, HeLa, and HepG2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-VPS26A in 2.5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody [NBP2-75922] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-VPS26A Antibody NBP2-75922 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/VPS26A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75922-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-75922] - IHC analysis of a formalin fixed paraffin embedded tissue section of the mouse testis using 1:350 dilution of VPS26A antibody (NBP2-75922). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunohistochemistry-Paraffin-NBP2-75922-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-VPS26A monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % inten](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Western-Blot-NBP2-36754-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U251 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0011.jpg)
![Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Western-Blot-NBP2-36754-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in HeLa cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human cell line A431 showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in MCF7 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U2OS cell line with Anti-VSP26A monoclonal antibody, showing distinct staining of endosomes in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0013.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0014.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0015.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0016.jpg)
![Western Blot: VPS26 Antibody [NBP2-49257] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/VPS26-Antibody-Western-Blot-NBP2-49257-img0002.jpg "Western Blot: VPS26 Antibody [NBP2-49257] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: VPS26 Antibody [NBP2-49257] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49257-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-49257] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunohistochemistry-Paraffin-NBP2-49257-img0009.jpg)
![Immunohistochemistry-Paraffin: VPS26 Antibody [NBP2-49257] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunohistochemistry-Paraffin-NBP2-49257-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VPS26A Antibody [NBP2-49257] - VPS26 Antibody [NBP2-49257] - Staining in human testis and pancreas tissues using anti-VPS26A antibody. Corresponding VPS26A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VPS26-Antibody-Immunohistochemistry-Paraffin-NBP2-49257-img0010.jpg)
![Western Blot: VPS26A Antibody [NBP2-93897] - Analysis of extracts of various cell lines, using VPS26A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/VPS26A-Antibody-Western-Blot-NBP2-93897-img0002.jpg "Western Blot: VPS26A Antibody [NBP2-93897] - Analysis of extracts of various cell lines, using VPS26A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody [NBP2-93897] - Analysis of NIH-3T3 cells using VPS26A . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VPS26A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93897-img0001.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody [NBP2-97752] - Immunochemical staining of human VPS26A in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/VPS26A-Antibody-Immunohistochemistry-Paraffin-NBP2-97752-img0001.jpg "Immunohistochemistry-Paraffin: VPS26A Antibody [NBP2-97752] - Immunochemical staining of human VPS26A in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")