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USF2 Antibodies

We offer USF2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each USF2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our USF2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the USF2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our USF2 polyclonal antibodies and browse our USF2 monoclonal antibody catalog.

Alternate Names for USF2 Antibodies

anti-USF2 antibody, anti-BHLHB12 antibody, anti-bHLHb12upstream stimulatory factor 2 antibody, anti-Class B basic helix-loop-helix protein 12 antibody, anti-FIPc-fos interacting protein antibody, anti-FOS-interacting protein antibody, anti-Major late transcription factor 2 antibody, anti-Upstream transcription factor 2 antibody, anti-upstream transcription factor 2, c-fos interacting antibody

11 Results for "usf2" in Primary Antibodies

NBP1-92649

USF2 Antibody

(3 Publications)

100 ul

NBP1-92649

Western Blot: USF2 Antibody [NBP1-92649] - Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92649 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92647 and NBP1-92650, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry-Paraffin: USF2 Antibody [NBP1-92649] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunohistochemistry-Paraffin: USF2 Antibody [NBP1-92649] - FFPE serial sections of human prostate carcinoma.Antibody: Affinity purified rabbit anti- USF2 left image, middle image and right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

<b>Genetic Strategies Validation. </b>Knockout: USF2 Antibody [NBP1-92649] - USF2 depletion in MLLr leukemia cells. Q-PCR was performed to validate the transcriptional regulation of HOXA9 upon USF2 targeting by two sgRNAs (sgUSF2#2 and sgUSF2#3) in OCI-AML2 cells. Immunoblotting confirmed the complete depletion of USF2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33001025/) licensed under a CC-BY license.

Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). A. Detection by RPPA of VCL and ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 and VCL protein and Pearson correlation between dilution and the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following open publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction(A) Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1–5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1–5) with homozygous precise correction of the ASXL1 mutation. beta -actin was used as loading control. (B) Evaluation of the expression levels of HOXA genes using quantitative real-time PCR (q-RT-PCR). The expression levels of HOXA5, 6, 7, 9, 10 and 13 were measured in three ASXL1 homozygous corrected KBM5 clones compared with uncorrected cells. The results shown were obtained from six independent experiments for each clone. The values in ASXL1 homozygous corrected cells are relative to the uncorrected cells. Bar graphs show mean + standard error of the mean (s.e.m.) (* = P 500 cells). (e,f) Cells were transfected with pEGFP-2B or pEGFP-T beta 4 plasmid for 24 h. (e) The expression of GFP and T beta 4-GFP were detected with GFP antibody. (f) The cells were fixed and stained with antibody against Ac-tubulin (red) and DAPI (blue). The ciliated cells in GFP (white) or T beta 4-GFP-positive cells (grey) were counted. Processing (such as changing brightness and contrast) is applied equally to controls across the entire image. Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

A. Gene ontology analysis of proteins identified by CASID targeting telomeres. B. Western blot detection of TRF1, POT1, and DSP in the proteins pulled down by CASID with the presence of telo-sgRNA in HEK293 cells. Input represents the initial nuclear extract before the pull-down mediated by CASID. C. left, ChIP assay of telomere binding of TRF2 or DSP in MCF-7 cells and HEK293 cells. The chromatin DNA precipitated by anti-DSP and anti-TRF2 antibodies were analyzed by dot blot using the probes recognizing telomere (Telo-p) or Alu DNA repeats (Alu-p). The detection of Alu DNA repeats serves as negative control. The dot-blot was performed at least three times and the represented one is shown. Right, the histogram shows the quantitated results of the dot blots. The triple asterisks (***) indicates a p-value less than 0.01. D. Fluorescence imaging of DSP-EGFP in MCF-7 cells and U2OS cells. The EGFP signal indicates the localization of DSP-EGFP (green) and the Telo-FISH signal indicates the localization of telomeres (red). The overlapping between DSP-EGFP and Telo-FISH is in yellow as revealed in the enlarged nuclear area. The dCAS9-EGFP construct without telo-sgRNA was used for U2OS transfection and the imaging of EGFP serves as negative control. E. DSP localization in interphase and pro-metaphase nucleus. DSP-EGFP indicates the DSP locations and DAPI indicates the nucleus. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31595153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92649AF350
Alexa Fluor 405	NBP1-92649AF405
Alexa Fluor 488	NBP1-92649AF488
Alexa Fluor 532	NBP1-92649AF532
Alexa Fluor 594	NBP1-92649AF594
Alexa Fluor 647	NBP1-92649AF647
Alexa Fluor 700	NBP1-92649AF700
Alexa Fluor 750	NBP1-92649AF750
Allophycocyanin	NBP1-92649APC
Biotin	NBP1-92649B
DyLight 350	NBP1-92649UV
DyLight 405	NBP1-92649V
DyLight 488	NBP1-92649G
DyLight 550	NBP1-92649R
DyLight 594	NBP1-92649DL594
DyLight 650	NBP1-92649C
DyLight 680	NBP1-92649FR
DyLight 755	NBP1-92649IR
FITC	NBP1-92649F
HRP	NBP1-92649H
Janelia Fluor 549	NBP1-92649JF549
Janelia Fluor 646	NBP1-92649JF646
PE	NBP1-92649PE
PerCP	NBP1-92649PCP

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H00007392-M01

USF2 Antibody (5E9)

(1 Publication)

0.1 mg

H00007392-M01

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in different cell lines.

Immunocytochemistry/Immunofluorescence: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1 ug/ml.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in PC-12.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in Hela S3 NE.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - Analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody (M01), clone 5E9.Lane 1: USF2 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: USF2 Antibody (5E9) [H00007392-M01] - Detection limit for recombinant GST tagged USF2 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00007392-M02

USF2 Antibody (5F2)

0.1 mg

H00007392-M02

Western Blot: USF2 Antibody (5F2) [H00007392-M02] - USF2 monoclonal antibody (M02), clone 5F2. Analysis of USF2 expression in different cell lines.

Immunocytochemistry/Immunofluorescence: USF2 Antibody (5F2) [H00007392-M02] - Analysis of monoclonal antibody to USF2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: USF2 Antibody (5F2) [H00007392-M02] - Analysis of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1 ug/ml.

Western Blot: USF2 Antibody (5F2) [H00007392-M02] - USF2 monoclonal antibody (M02), clone 5F2. Analysis of USF2 expression in PC-12.

Sandwich ELISA: USF2 Antibody (5F2) [H00007392-M02] - Detection limit for recombinant GST tagged USF2 is approximately 10ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00007392-M03

USF2 Antibody (6A9)

(1 Publication)

0.1 mg

H00007392-M03

Western Blot: USF2 Antibody (6A9) [H00007392-M03] - Analysis of USF2 expression in PC-12 (Cat # L012V1).

Immunocytochemistry/Immunofluorescence: USF2 Antibody (6A9) [H00007392-M03] - Analysis of monoclonal antibody to USF2 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: USF2 Antibody (6A9) [H00007392-M03] - Western Blot analysis of USF2 expression in different cell lines.

Sandwich ELISA: USF2 Antibody (6A9) [H00007392-M03] - Detection limit for recombinant GST tagged USF2 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-92647

USF2 Antibody

100 ul

NBP1-92647

Immunohistochemistry: USF2 Antibody [NBP1-92647] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunohistochemistry: USF2 Antibody [NBP1-92647] - Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunoprecipitation: USF2 Antibody [NBP1-92647] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92647 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92650, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92647AF350
Alexa Fluor 405	NBP1-92647AF405
Alexa Fluor 488	NBP1-92647AF488
Alexa Fluor 532	NBP1-92647AF532
Alexa Fluor 594	NBP1-92647AF594
Alexa Fluor 647	NBP1-92647AF647
Alexa Fluor 700	NBP1-92647AF700
Alexa Fluor 750	NBP1-92647AF750
Allophycocyanin	NBP1-92647APC
Biotin	NBP1-92647B
DyLight 350	NBP1-92647UV
DyLight 405	NBP1-92647V
DyLight 488	NBP1-92647G
DyLight 550	NBP1-92647R
DyLight 594	NBP1-92647DL594
DyLight 650	NBP1-92647C
DyLight 680	NBP1-92647FR
DyLight 755	NBP1-92647IR
FITC	NBP1-92647F
HRP	NBP1-92647H
Janelia Fluor 549	NBP1-92647JF549
Janelia Fluor 646	NBP1-92647JF646
PE	NBP1-92647PE
PerCP	NBP1-92647PCP

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NBP3-12887

USF2 Antibody

100 ul

NBP3-12887

Western Blot: USF2 Antibody [NBP3-12887] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with USF2 antibody (NBP3-12887) diluted at 1:500 and competitor's antibody diluted by 1:200.

<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: USF2 Antibody [NBP3-12887] - USF2 antibody detects USF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USF2 stained by USF2 antibody (NBP3-12887) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.

Western Blot: USF2 Antibody [NBP3-12887] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USF2 antibody (NBP3-12887) diluted at 1:500.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-92650

USF2 Antibody

100 ul

NBP1-92650

Immunohistochemistry: USF2 Antibody [NBP1-92650] - Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunoprecipitation: USF2 Antibody [NBP1-92650] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92647 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92650, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

<b>Independent Antibodies Validation. </b>Immunoprecipitation: USF2 Antibody [NBP1-92650] - Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92650 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92647, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92650AF350
Alexa Fluor 405	NBP1-92650AF405
Alexa Fluor 488	NBP1-92650AF488
Alexa Fluor 532	NBP1-92650AF532
Alexa Fluor 594	NBP1-92650AF594
Alexa Fluor 647	NBP1-92650AF647
Alexa Fluor 700	NBP1-92650AF700
Alexa Fluor 750	NBP1-92650AF750
Allophycocyanin	NBP1-92650APC
Biotin	NBP1-92650B
DyLight 350	NBP1-92650UV
DyLight 405	NBP1-92650V
DyLight 488	NBP1-92650G
DyLight 550	NBP1-92650R
DyLight 594	NBP1-92650DL594
DyLight 650	NBP1-92650C
DyLight 680	NBP1-92650FR
DyLight 755	NBP1-92650IR
FITC	NBP1-92650F
HRP	NBP1-92650H
Janelia Fluor 549	NBP1-92650JF549
Janelia Fluor 646	NBP1-92650JF646
PE	NBP1-92650PE
PerCP	NBP1-92650PCP

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NBP2-56717

USF2 Antibody

(1 Publication)

100 ul

NBP2-56717

Immunocytochemistry/Immunofluorescence: USF2 Antibody [NBP2-56717] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-23753

USF2 Antibody (PCRP-USF2-1A7)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-24186AF350
Alexa Fluor 405	NBP3-24186AF405
Alexa Fluor 488	NBP3-24186AF488
Alexa Fluor 532	NBP3-24186AF532
Alexa Fluor 594	NBP3-24186AF594
Alexa Fluor 647	NBP3-24186AF647
Alexa Fluor 700	NBP3-24186AF700
Alexa Fluor 750	NBP3-24186AF750
Allophycocyanin	NBP3-24186APC
Biotin	NBP3-24186B
DyLight 350	NBP3-24186UV
DyLight 405	NBP3-24186V
DyLight 488	NBP3-24186G
DyLight 550	NBP3-24186R
DyLight 594	NBP3-24186DL594
DyLight 650	NBP3-24186C
DyLight 680	NBP3-24186FR
DyLight 755	NBP3-24186IR
FITC	NBP3-24186F
HRP	NBP3-24186H
Janelia Fluor 549	NBP3-24186JF549
Janelia Fluor 646	NBP3-24186JF646
PE	NBP3-24186PE
PerCP	NBP3-24186PCP
mFluor Violet 450 SE	NBP3-24186MFV450
mFluor Violet 500 SE	NBP3-24186MFV500
mFluor Violet 610 SE	NBP3-24186MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-24186

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NBP2-58499

USF2 Antibody

100 ul

NBP2-58499

Immunocytochemistry/Immunofluorescence: USF2 Antibody [NBP2-58499] - Staining of human cell line A-431 shows localization to nucleoplasm.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-92650AF350

USF2 Antibody [Alexa Fluor® 350]

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Alexa Fluor 350

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NBP1-92650AF405
Alexa Fluor 488	NBP1-92650AF488
Alexa Fluor 532	NBP1-92650AF532
Alexa Fluor 594	NBP1-92650AF594
Alexa Fluor 647	NBP1-92650AF647
Alexa Fluor 700	NBP1-92650AF700
Alexa Fluor 750	NBP1-92650AF750
Allophycocyanin	NBP1-92650APC
Biotin	NBP1-92650B
DyLight 350	NBP1-92650UV
DyLight 405	NBP1-92650V
DyLight 488	NBP1-92650G
DyLight 550	NBP1-92650R
DyLight 594	NBP1-92650DL594
DyLight 650	NBP1-92650C
DyLight 680	NBP1-92650FR
DyLight 755	NBP1-92650IR
FITC	NBP1-92650F
HRP	NBP1-92650H
Janelia Fluor 549	NBP1-92650JF549
Janelia Fluor 646	NBP1-92650JF646
PE	NBP1-92650PE
PerCP	NBP1-92650PCP

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