UBA3 Antibodies
We offer UBA3 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each UBA3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our UBA3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the UBA3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our UBA3 polyclonal antibodies and browse our UBA3 monoclonal antibody catalog.
Alternate Names for UBA3 Antibodies
anti-UBA3 antibody, anti-DKFZp566J164 antibody, anti-EC 6.3.2 antibody, anti-EC 6.3.2.- antibody, anti-hUBA3 antibody, anti-MGC22384 antibody, anti-NEDD8-activating enzyme E1 catalytic subunit antibody, anti-NEDD8-activating enzyme E1C antibody, anti-Nedd8-activating enzyme hUba3 antibody, anti-UBA3, ubiquitin-activating enzyme E1 homolog antibody, anti-UBE1C antibody, anti-Ubiquitin-activating enzyme 3 antibody, anti-ubiquitin-activating enzyme E1C (homologous to yeast UBA3) antibody, anti-Ubiquitin-activating enzyme E1C antibody, anti-ubiquitin-like modifier activating enzyme 3 antibody, anti-Ubiquitin-like modifier-activating enzyme 3 antibody, anti-yeast) antibody
9 Results for "uba3" in Primary Antibodies
![Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-48628-img0002.jpg "Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0007.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0005.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human skin shows moderate nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-48628-img0006.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining in human skin and pancreas tissues using anti-UBA3 antibody. Corresponding UBA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: UBA3 Antibody [NBP2-49443] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-49443-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UBA3 Antibody [NBP2-49443] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & centrosome.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49443-img0004.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0006.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-49443] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP2-49443-img0005.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-28820241419157.jpg "Western Blot: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33074) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HL-60 cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33074) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1C10-5-5-R) [NBP3-33074] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-UBA3 antibody (NBP3-33074) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33074_mouse-uba3-mab-1c10-5-5-r-298202414262662.jpg)
![Immunohistochemistry-Paraffin: UBA3 Antibody [NBP1-83150] - staining of human lateral ventricle shows strong nuclear and additional cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/UBA3-Antibody-Immunohistochemistry-Paraffin-NBP1-83150-img0007.jpg "Immunohistochemistry-Paraffin: UBA3 Antibody [NBP1-83150] - staining of human lateral ventricle shows strong nuclear and additional cytoplasmic positivity in neuronal cells.")
![Western Blot: UBA3 Antibody (S05-6C8) [NBP3-20008] - Western blot detection of UBA3 in Hela, A549, HL-60 cell lysates using NBP3-20008 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/UBA3-Antibody-S05-6C8-Western-Blot-NBP3-20008-img0001.jpg "Western Blot: UBA3 Antibody (S05-6C8) [NBP3-20008] - Western blot detection of UBA3 in Hela, A549, HL-60 cell lysates using NBP3-20008 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33073_mouse-uba3-mab-1b6-6-5-r-28820241419156.jpg "Western Blot: UBA3 Antibody (1B6-6-5-R) [NBP3-33073] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33073) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/UBA3-Antibody-Western-Blot-NBP2-20777-img0001.jpg "Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.")