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![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0018.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.")
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat olfactory bulb at 1:500 dilution using Rabbit antibody to TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98844-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0002.jpg)
![Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-Flow-Cytometry-NBP2-62000-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-62000-img0004.jpg)
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-62000-img0001.jpg)
![Western Blot: TRPC1 Antibody [NBP2-50090] - WB on mouse brain lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/TRP1-Antibody-Western-Blot-NBP2-50090-img0002.jpg "Western Blot: TRPC1 Antibody [NBP2-50090] - WB on mouse brain lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-50090] - IHC-P on paraffin section of mouse cerebellum.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-50090-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP1-89648] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89648-img0003.jpg "Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP1-89648] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.")
![Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP1-89648] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.](http://images.novusbio.com/fullsize/TRP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89648-img0004.jpg)
![Western Blot: TRPC1 Antibody (1V3Q2) [NBP3-16315] - Western blot analysis of extracts of various cell lines, using TRPC1 Rabbit mAb (NBP3-16315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/TRPC1-Antibody-1V3Q2-Western-Blot-NBP3-16315-img0001.jpg "Western Blot: TRPC1 Antibody (1V3Q2) [NBP3-16315] - Western blot analysis of extracts of various cell lines, using TRPC1 Rabbit mAb (NBP3-16315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: TRPC1 Antibody (SR1100) [NBP3-22029] - Western blot analysis using NBP3-22029 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22029_rabbit-trpc1-mab-sr1100-57202316494221.jpg "Western Blot: TRPC1 Antibody (SR1100) [NBP3-22029] - Western blot analysis using NBP3-22029 in HepG2 cell lysate.")
![Western Blot: TRPC1 Antibody (JE35-25) [NBP3-33060] - Western blot analysis of TRPC1 on different lysates with Rabbit anti-TRPC1 antibody (NBP3-33060) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate 15 ug/Lane) Lane 2: HepG2 cell lysate 15 ug/Lane) Lane 3: A549 cell lysate 15 ug/Lane) Lane 4: MCF7 cell lysate 15 ug/Lane) Lane 5: SK-OV-3 cell lysate 15 ug/Lane) Lane 6: NIH/3T3 cell lysate 15 ug/Lane) Lane 7: PC-12 cell lysate 15 ug/Lane) Lane 8: Human brain tissue lysate (30 ug/Lane) Lane 9: Mosue brain tissue lysate (30 ug/Lane) Lane 10: Mosue testis tissue lysate (30 ug/Lane) Lane 11: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 91 kDa Observed band size: 120 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33060_rabbit-trpc1-mab-je35-25-288202412493427.jpg "Western Blot: TRPC1 Antibody (JE35-25) [NBP3-33060] - Western blot analysis of TRPC1 on different lysates with Rabbit anti-TRPC1 antibody (NBP3-33060) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate 15 ug/Lane) Lane 2: HepG2 cell lysate 15 ug/Lane) Lane 3: A549 cell lysate 15 ug/Lane) Lane 4: MCF7 cell lysate 15 ug/Lane) Lane 5: SK-OV-3 cell lysate 15 ug/Lane) Lane 6: NIH/3T3 cell lysate 15 ug/Lane) Lane 7: PC-12 cell lysate 15 ug/Lane) Lane 8: Human brain tissue lysate (30 ug/Lane) Lane 9: Mosue brain tissue lysate (30 ug/Lane) Lane 10: Mosue testis tissue lysate (30 ug/Lane) Lane 11: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 91 kDa Observed band size: 120 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![ELISA: TRPC1 Antibody (1E4) [H00007220-M03-50ug] - Detection limit for recombinant GST tagged TRPC1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-1E4-ELISA-H00007220-M03-50ug-img0001.jpg "ELISA: TRPC1 Antibody (1E4) [H00007220-M03-50ug] - Detection limit for recombinant GST tagged TRPC1 is 0.1 ng/ml as a capture antibody.")
![ELISA: TRPC1 Antibody (1E4) [H00007220-M03-50ug] - Detection limit for recombinant GST tagged TRPC1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRP1-Antibody-1E4-ELISA-H00007220-M03-50ug-img0002.jpg)