TMEM49 Antibodies
Transmembrane protein 49 (TMEM49), also known as vacuole membrane protein 1 (VMP1), is an endoplasmic reticulum (ER)-localized protein that is plays a critical role in the process of autophagy. TMEM49/VMP1 is a 406 amino acid transmembrane protein with a VTT domain and with a theoretical molecular weight of 46 kDa. Browse our catalog of polyclonal and TMEM49-conjugated antibodies. Common research applications for our TMEM49 antibodies include ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, and Western Blot. Our TMEM49 antibodies are reactive in many species such as Human, Mouse, Rat, Bovine, Equine, Guinea Pig, and Rabbit. Choose the anti-TMEM49 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each TMEM49 antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it.
Alternate Names for TMEM49 Antibodies
anti-TMEM49 antibody, anti-VMP1 antibody, anti-DKFZp566I133 antibody, anti-EPG3 antibody, anti-TDC1 antibody, anti-transmembrane protein 49TMEM49 antibody, anti-vacuole membrane protein 1 antibody
6 Results for "tmem49" in Primary Antibodies
![Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-78784-img0003.jpg "Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TMEM49 Antibody [NBP2-78784] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TMEM49 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78784-img0001.jpg)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP2-78784] - Analysis of a FFPE tissue section of the human testis using 1:200 dilution of TMEM49 antibody (NBP2-78784). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in cytoplasm and membrane in seminiferous tubules.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP2-78784-img0002.jpg)
![Western Blot: TMEM49 Antibody [NBP2-93149] - Analysis of extracts of mouse kidney, using TMEM49 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-93149-img0003.jpg "Western Blot: TMEM49 Antibody [NBP2-93149] - Analysis of extracts of mouse kidney, using TMEM49 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: TMEM49 Antibody [NBP2-93149] - Analysis of NIH-3T3 cells using TMEM49 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93149-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: TMEM49 Antibody - Azide and BSA Free [NBP2-93149] - Immunofluorescence analysis of U-2 OS cells using TMEM49 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93149_rabbit-polyclonal-tmem49-antibody-4220251431818.jpg%3Ft=1709173921)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP1-83971] - Staining of human liver shows negative to very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP1-83971-img0004.jpg "Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP1-83971] - Staining of human liver shows negative to very weak cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP1-83971] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP1-83971-img0001.jpg)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP1-83971] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP1-83971-img0002.jpg)
![Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP1-83971] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TMEM49-Antibody-Immunohistochemistry-Paraffin-NBP1-83971-img0003.jpg)
![Western Blot: TMEM49 Antibody [NBP2-88442] - Host: Rabbit. Target Name: TMEM49. Sample Tissue: Mouse RAW264.7 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-88442-img0001.jpg "Western Blot: TMEM49 Antibody [NBP2-88442] - Host: Rabbit. Target Name: TMEM49. Sample Tissue: Mouse RAW264.7 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: TMEM49 Antibody [NBP2-88441] - Host: Rabbit. Target Name: VMP1. Sample Type: HepG2 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/TMEM49-Antibody-Western-Blot-NBP2-88441-img0001.jpg "Western Blot: TMEM49 Antibody [NBP2-88441] - Host: Rabbit. Target Name: VMP1. Sample Type: HepG2 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")