SP100 Antibodies
We offer SP100 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each SP100 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SP100 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the SP100 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SP100 polyclonal antibodies and browse our SP100 monoclonal antibody catalog.
Alternate Names for SP100 Antibodies
anti-SP100 antibody, anti-DKFZp686E07254 antibody, anti-FLJ00340 antibody, anti-FLJ34579 antibody, anti-lysp100b antibody, anti-nuclear antigen Sp100 antibody, anti-nuclear autoantigen Sp-100 antibody, anti-Nuclear dot-associated Sp100 protein antibody, anti-SP100 nuclear antigen antibody, anti-SP100-HMG nuclear autoantigen antibody, anti-Speckled 100 kDa antibody
15 Results for "sp100" in Primary Antibodies
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0018.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP1-86060-img0006.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0021.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0019.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0020.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunocytochemistry-Immunofluorescence-NBP3-13780-img0002.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0004.jpg)
![Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Flow cytometric analysis of PFA-fixed HeLa cells. SP100 antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Flow-Cytometry-NBP3-13780-img0001.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0003.jpg)
![Protein Array: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Antibody (PCRP-SP100-1B9).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Protein-Array-NBP3-13780-img0006.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-89457 (A) shows similar protein distribution across tissues to independent antibody NBP1-86060 (B).](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-89457 (A) shows similar protein distribution across tissues to independent antibody NBP1-86060 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP1-89457] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89457-img0004.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0016.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0014.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0015.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0017.jpg)
![Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in mouse kidney.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-H00006672-D01P-img0003.jpg "Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in mouse kidney.")
![Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in transfected 293T cell line by SP100 polyclonal antibody.Lane 1: SP100 transfected lysate(53.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-H00006672-D01P-img0004.jpg)
![Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0005.jpg "Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 over-expressed 293 cell line, cotransfected with SP100 Validated Chimera RNAi ( Cat # H00006672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP100 monoclonal antibody (M02), clone 1G6 (Cat # H00006672-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0003.jpg)
![Sandwich ELISA: SP100 Antibody (1G6) [H00006672-M02] - Detection limit for recombinant GST tagged SP100 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Sandwich-ELISA-H00006672-M02-img0004.jpg)
![Western Blot: SP100 Antibody [NBP3-13031] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP100 antibody (NBP3-13031) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP3-13031-img0002.jpg "Western Blot: SP100 Antibody [NBP3-13031] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP100 antibody (NBP3-13031) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP3-13031] - SP100 antibody detects SP100 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 antibody (NBP3-13031) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13031-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97408] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97408-img0001.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97408] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408] - Immunochemical staining of human SP100 in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP2-97408-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97407] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97407-img0001.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97407] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97407] - Immunochemical staining of human SP100 in human gallbladder with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP2-97407-img0002.jpg)
![Western Blot: SP100 Antibody [NBP3-10281] - Western blot analysis of SP100 in Mouse Heart lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP3-10281-img0001.jpg "Western Blot: SP100 Antibody [NBP3-10281] - Western blot analysis of SP100 in Mouse Heart lysates. Antibody dilution at 1ug/ml")
![Western Blot: SP100 Antibody [NBP2-99036] - Anti-SP100 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99036_rabbit-polyclonal-sp100-antibody-western-blot-2182023113444..jpg "Western Blot: SP100 Antibody [NBP2-99036] - Anti-SP100 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 95 kDa")
![ELISA: SP100 Antibody (2E2) [H00006672-M03] - Detection limit for recombinant GST tagged SP100 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-2E2-ELISA-H00006672-M03-img0001.jpg "ELISA: SP100 Antibody (2E2) [H00006672-M03] - Detection limit for recombinant GST tagged SP100 is approximately 0.3ng/ml as a capture antibody.")