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Smad2/3 Antibodies

We offer SMAD2/3 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each SMAD2/3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SMAD2/3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the SMAD2/3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SMAD2/3 polyclonal antibodies.

Alternate Names for Smad2/3 Antibodies

anti-Smad2/3 antibody, anti-SMAD2 antibody, anti-SMAD3 antibody, anti-hMAD-2 antibody, anti-hSMAD2 antibody, anti-JV18 antibody, anti-JV18-1SMAD, mothers against DPP homolog 2 (Drosophila) antibody, anti-MAD homolog 2 antibody, anti-Mad protein homolog antibody, anti-Mad, mothers against decapentaplegic homolog 2 antibody, anti-Mad-related protein 2 antibody, anti-MADH2MGC34440 antibody, anti-MADR2MGC22139 antibody, anti-mother against DPP homolog 2 antibody, anti-mothers against decapentaplegic homolog 2 antibody, anti-Mothers against DPP homolog 2 antibody, anti-Sma- and Mad-related protein 2 antibody, anti-SMAD 2 antibody, anti-SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila) antibody, anti-SMAD, mothers against DPP homolog 2 antibody

3 Results for "smad2-3" in Primary Antibodies

AF3797

Smad2/3 Antibody [Unconjugated]

(1 Review)

(24 Publications)

100 μg

AF3797

Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.

Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad2/3 at approximately 64 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3797-SP

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MAB8935

Smad2/3 [p Ser465, p Ser467] Antibody (1074A) [Unconjugated]

(11 Publications)

100 μg

MAB8935

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8935-SP

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NBP3-15589

Smad2/3 Antibody - Azide and BSA Free

100 ul

NBP3-15589

Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-15589-20ul

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