Smad1 Antibodies
We offer Smad1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each Smad1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Smad1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the Smad1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Smad1 polyclonal antibodies and browse our Smad1 monoclonal antibody catalog.
Alternate Names for Smad1 Antibodies
anti-Smad1 antibody, anti-BSP-1 antibody, anti-BSP1 antibody, anti-hSMAD1 antibody, anti-JV4-1SMAD, mothers against DPP homolog 1 (Drosophila) antibody, anti-MAD homolog 1 antibody, anti-Mad-related protein 1 antibody, anti-MADH1JV41 antibody, anti-MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila) antibody, anti-mothers against decapentaplegic homolog 1 antibody, anti-Mothers against DPP homolog 1 antibody, anti-SMAD family member 1SMAD 1 antibody, anti-SMAD, mothers against DPP homolog 1 antibody, anti-TGF-beta signaling protein 1 antibody, anti-transforming growth factor-beta signaling protein 1 antibody
12 Results for "smad1" in Primary Antibodies
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0025.jpg "Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Immunocytochemistry-Immunofluorescence-H00004086-M03-img0018.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Immunohistochemistry-Paraffin-H00004086-M03-img0020.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0021.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0022.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in IMR-32 (Cat # L008V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0023.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0024.jpg)
![ELISA: Smad1 Antibody (2E9) [H00004086-M03] - Detection limit for recombinant GST tagged SMAD1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-ELISA-H00004086-M03-img0009.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.Lane 1: SMAD1 transfected lysate(52.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0023.jpg "Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.Lane 1: SMAD1 transfected lysate(52.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Immunocytochemistry-Immunofluorescence-H00004086-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Immunohistochemistry-Paraffin-H00004086-M02-img0018.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3. Analysis of SMAD1 expression in A-549.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0020.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3 Analysis of SMAD1 expression in IMR-32.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0021.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3. Analysis of SMAD1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0022.jpg)
![Sandwich ELISA: Smad1 Antibody (1D3) [H00004086-M02] - Detection limit for recombinant GST tagged SMAD1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Sandwich-ELISA-H00004086-M02-img0019.jpg)
![Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Western-Blot-NBP3-15670-img0006.jpg "Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad1 Rabbit mAb (NBP3-15670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunocytochemistry-Immunofluorescence-NBP3-15670-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0004.jpg)
![Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of various cell lines, using Smad1 antibody (NBP3-15670) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Western-Blot-NBP3-15670-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded mouse kidney using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts from normal (control) and Smad1 knockout (KO) HeLa cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Knockout-Validated-NBP3-15670-img0001.jpg)
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0017.jpg "Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Immunocytochemistry-Immunofluorescence-H00004086-M04-img0012.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Immunohistochemistry-Paraffin-H00004086-M04-img0014.jpg)
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0015.jpg)
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0016.jpg)
![Western Blot: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Western-Blot-NBP2-45869-img0004.jpg "Western Blot: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.")
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0003.jpg)
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0001.jpg)
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0002.jpg)
![Western Blot: Smad1 Antibody (OTI1C5) - Azide and BSA Free [NBP2-74236] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.](http://images.novusbio.com/fullsize/Smad1-Antibody-OTI1C5-Azide-and-BSA-Free-Western-Blot-NBP2-74236-img0001.jpg "Western Blot: Smad1 Antibody (OTI1C5) - Azide and BSA Free [NBP2-74236] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.")
![Immunohistochemistry: Smad1 Antibody (OTI1C5) - Azide and BSA Free [NBP2-74236] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-OTI1C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74236-img0003.jpg)
![Immunohistochemistry: Smad1 Antibody (OTI1C5) - Azide and BSA Free [NBP2-74236] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-OTI1C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74236-img0002.jpg)
![Immunohistochemistry: Smad1 Antibody (OTI1C5) - Azide and BSA Free [NBP2-74236] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-OTI1C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74236-img0004.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0008.jpg "Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0005.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0006.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0007.jpg)
![Western Blot: Smad1 [p Ser206] Antibody [NBP1-77860] - This antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX](http://images.novusbio.com/fullsize/Smad1-[p-Ser206]-Antibody-Western-Blot-NBP1-77860-img0004.jpg "Western Blot: Smad1 [p Ser206] Antibody [NBP1-77860] - This antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX")
![Western Blot: Smad1 [p Ser206] Antibody [NBP1-77858] - Shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ug/ml), EGF and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reaction with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.](http://images.novusbio.com/fullsize/Smad1-[p-Ser206]-Antibody-Western-Blot-NBP1-77858-img0003.jpg "Western Blot: Smad1 [p Ser206] Antibody [NBP1-77858] - Shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ug/ml), EGF and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reaction with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.")
![Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/Smad1-p-Ser463-p-Ser465-Antibody-S07-4A3-Western-Blot-NBP3-15092-img0001.jpg "Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")