Skp2 Antibodies
We offer SKP2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot. Each SKP2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SKP2 Antibodies can be used in a variety of model species: Human. Use the list below to choose the SKP2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SKP2 polyclonal antibodies and browse our SKP2 monoclonal antibody catalog.
Alternate Names for Skp2 Antibodies
anti-Skp2 antibody, anti-CDK2/cyclin A-associated protein p45 antibody, anti-cyclin A/CDK2-associated protein p45 antibody, anti-Cyclin-A/CDK2-associated protein p45 antibody, anti-F-box protein Skp2 antibody, anti-F-box/LRR-repeat protein 1 antibody, anti-FBL1 antibody, anti-FBXL1MGC1366 antibody, anti-FLB1 antibody, anti-p45skp2 antibody, anti-S-phase kinase-associated protein 2 (p45) antibody, anti-S-phase kinase-associated protein 2 antibody
10 Results for "skp2" in Primary Antibodies
![Western Blot: Skp2 Antibody [NBP2-49142] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-49142-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP2-49142] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0008.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0005.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0006.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0007.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0002.jpg "Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Skp2 Antibody (2L0K1) [NBP3-16320] - Immunohistochemistry of paraffin-embedded human colon using Skp2 Rabbit mAb (NBP3-16320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Immunohistochemistry-Paraffin-NBP3-16320-img0001.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of various cell lines, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0003.jpg)
![Western Blot: Skp2 Antibody [NBP2-56192] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-56192-img0002.jpg "Western Blot: Skp2 Antibody [NBP2-56192] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-56192] - Staining of human cell line PC-3 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56192-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP2-56192] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-56192-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203] - Immunofluorescence staining of Hela Cells with at Skp2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Skp2-Antibody-6D4-Immunocytochemistry-Immunofluorescence-NBP3-15203-img0001.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203] - Immunofluorescence staining of Hela Cells with at Skp2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry: Skp2 Antibody (6D4) [NBP3-15203] - IHC image of Skp2 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Skp2-Antibody-6D4-Immunohistochemistry-NBP3-15203-img0002.jpg)
![Western Blot: Skp2 Antibody [NBP1-30077] - Detection of Human SKP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP1-30077-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP1-30077] - Detection of Human SKP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Skp2 Antibody [NBP1-30077] - Detection of human SKP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for IP at 6 ug per reaction. SKP2 was also immunoprecipitated by another rabbit anti-SKP2 antibody. For blotting immunoprecipitated SKP2, NBP1-30077 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunoprecipitation-NBP1-30077-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-15197-img0002.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Skp2 Antibody (3C11) [NBP3-15197] - Overlay histogram showing Hela cells stained with Skp2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Flow-Cytometry-NBP3-15197-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (S08-0A2) [NBP3-19917] - Immunocytochemistry of Skp2 (green) in hela using NBP3-19917 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Skp2-Antibody-S08-0A2-Immunocytochemistry-Immunofluorescence-NBP3-19917-img0001.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (S08-0A2) [NBP3-19917] - Immunocytochemistry of Skp2 (green) in hela using NBP3-19917 at dilution 1/50, and DAPI (blue)")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Skp2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97674-img0001.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Skp2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")