RhoA Antibodies
We offer RhoA Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each RhoA Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RhoA Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the RhoA Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RhoA polyclonal antibodies and browse our RhoA monoclonal antibody catalog.
Alternate Names for RhoA Antibodies
anti-RhoA antibody, anti-h12 antibody, anti-oncogene RHO H12 antibody, anti-ras homolog gene family, member A antibody, anti-Rho cDNA clone 12 antibody, anti-RHO12 antibody, anti-RHOH12ARH12ARHAAplysia ras-related homolog 12 antibody, anti-small GTP binding protein RhoA antibody, anti-transforming protein RhoA antibody
20 Results for "rhoa" in Primary Antibodies
![Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.](http://images.novusbio.com/fullsize/RhoA-Antibody-Western-Blot-NB100-91273-img0034.jpg "Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0024.jpg)
![Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-NB100-91273-img0040.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0023.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0029.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0031.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0032.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0016.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on mouse brain (cryo section) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0035.jpg)
![Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Western-Blot-NBP2-22528-img0001.jpg "Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunoprecipitation-NBP2-22528-img0002.jpg)
![Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a RhoA/C monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Western-Blot-NBP2-22529-img0001.jpg "Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a RhoA/C monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3%% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of mouse liver tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a RhoA/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunoprecipitation-NBP2-22529-img0002.jpg)
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249181915.jpg%3Fv=1666706023 "Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.")
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916249.jpg%3Fv=1666706023)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Jurkat cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249181921.jpg%3Fv=1666706024)
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against HEK293 (1) and RhoA (AA: 1-190)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249141942.jpg%3Fv=1666706023)
![ELISA: RhoA Antibody (7E7H4) [NBP3-27177] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024917035.jpg%3Fv=1666706023)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915828.jpg%3Fv=1666706024)
![Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded gastric cancer tissues using RhoA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916244.jpg%3Fv=1666706024)
![Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded endometrial carcinoma tissues using RhoA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915822.jpg%3Fv=1666706026)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of K562 cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916223.jpg%3Fv=1666706024)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of HepG2 cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915836.jpg%3Fv=1666706023)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916248.jpg%3Fv=1666706023)
![Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Western-Blot-H00000387-M04-img0009.jpg "Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Immunocytochemistry-Immunofluorescence-H00000387-M04-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Immunohistochemistry-Paraffin-H00000387-M04-img0007.jpg)
![Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in HL-60 (Cat # L014V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Western-Blot-H00000387-M04-img0008.jpg)
![ELISA: RhoA Antibody (1B12) [H00000387-M04] - Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-ELISA-H00000387-M04-img0004.jpg)
![Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Western-Blot-H00000387-M05-img0009.jpg "Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RhoA Antibody (1C1) [H00000387-M05] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Immunohistochemistry-Paraffin-H00000387-M05-img0005.jpg)
![Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Western-Blot-H00000387-M05-img0008.jpg)
![Proximity Ligation Assay: RhoA Antibody (1C1) [H00000387-M05] - Analysis of protein-protein interactions between MAPK8 and RHOA. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-RHOA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Proximity-Ligation-Assay-H00000387-M05-img0006.jpg)
![ELISA: RhoA Antibody (1C1) [H00000387-M05] - Detection limit for recombinant GST tagged RHOA is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-ELISA-H00000387-M05-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15485-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NBP3-15485-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RhoA-Antibody-Knockout-Validated-NBP3-15485-img0001.jpg)
![Western Blot: RhoA Antibody (1C3) [H00000387-M08] - Analysis of RHOA expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Western-Blot-H00000387-M08-img0005.jpg "Western Blot: RhoA Antibody (1C3) [H00000387-M08] - Analysis of RHOA expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Immunocytochemistry-Immunofluorescence-H00000387-M08-img0003.jpg)
![Sandwich ELISA: RhoA Antibody (1C3) [H00000387-M08] - Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Sandwich-ELISA-H00000387-M08-img0004.jpg)
![Western Blot: RhoA Antibody (1A11) [H00000387-M03] - Analysis of RHOA expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-Western-Blot-H00000387-M03-img0003.jpg "Western Blot: RhoA Antibody (1A11) [H00000387-M03] - Analysis of RHOA expression in HeLa (Cat # L013V1).")
![ELISA: RhoA Antibody (1A11) [H00000387-M03] - Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-ELISA-H00000387-M03-img0002.jpg)
![Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23372_rabbit-rhoa-p-ser188-pab-1992023141013.jpg "Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).")
![Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Western-Blot-NBP2-90107-img0003.jpg "Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (103) [NBP2-90107] - Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90107-img0002.jpg)
![Flow Cytometry: RhoA Antibody (036) [NBP2-90105] - Flow cytometric analysis of Human RHOA expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human RHOA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/RhoA-Antibody-036-Flow-Cytometry-NBP2-90105-img0001.jpg "Flow Cytometry: RhoA Antibody (036) [NBP2-90105] - Flow cytometric analysis of Human RHOA expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human RHOA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")