RGS8 Antibodies
We offer RGS8 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Western Blot. Each RGS8 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RGS8 Antibodies can be used in a variety of model species: Human, Mouse, Rabbit, Rat. Use the list below to choose the RGS8 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RGS8 polyclonal antibodies.
Alternate Names for RGS8 Antibodies
anti-RGS8 antibody, anti-MGC119067 antibody, anti-MGC119068 antibody, anti-MGC119069 antibody, anti-regulator of G-protein signaling 8 antibody, anti-regulator of G-protein signalling 8 antibody
5 Results for "rgs8" in Primary Antibodies
![Western Blot: RGS8 Antibody [NBP2-20153] - Decreased steady-state levels of Rgs8 message and protein in BAC-Q72 mice.Western blot analyses indicate reduction of Rgs8 steady-state levels in cerebella of BAC-Q72 mice, but no change in BAC-Q22 mice when compared with wild-type mice. The blot is a representative Western blot of 3 independently performed experiments with 2 animals each per BAC line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP2-20153-img0002.jpg "Western Blot: RGS8 Antibody [NBP2-20153] - Decreased steady-state levels of Rgs8 message and protein in BAC-Q72 mice.Western blot analyses indicate reduction of Rgs8 steady-state levels in cerebella of BAC-Q72 mice, but no change in BAC-Q22 mice when compared with wild-type mice. The blot is a representative Western blot of 3 independently performed experiments with 2 animals each per BAC line. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182) licensed under a CC-BY license.")
![Western Blot: RGS8 Antibody [NBP2-20153] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP2-20153-img0001.jpg)
![Western Blot: RGS8 Antibody [NBP2-20153] - Overexpression of mutant ATXN2 in human SH-SY5Y cells recapitulates down-regulation of in vivo steady-state levels of Rgs8 in BAC-Q72 mice.Cells were transfected with plasmids encoding Flag-tagged cDNAs of human ATXN2 containing Q22 or Q58 or Q108 repeats. Forty-eight hrs post-transfection, cells were selected with hygromycin (40 µg/ml) for 5–7 days & hygromycin resistant cells were harvested as two aliquots. (A) Protein extracts were prepared from one aliquot & subjected to Western blot analyses to measure steady-state levels of RGS8. The blots were re-probed for beta -Actin as an internal loading control. (B) Quantitative RT-PCR analyses of synthesized cDNAs from the other aliquot demonstrate moderate reduction of RGS8 mRNA in cells expressing Flag-ATXN2-Q108. The data are means ± SD, *p<0.05. (C) Mutant ATXN2 specifically induces decrease of RGS8 expression. MYC-tagged RGS8 cDNA including 5’ & 3’ UTRs was cloned under the transcriptional control of the CMV promoter & transfected into short-term hygromycin selected SH-SY5Y cell lines expressing Flag-tagged ATXN2-Q22, -Q58 or -Q108. Forty-eight hrs post-transfection, levels of exogenous RGS8 are significantly decreased in cells expressing ATXN2-Q58 or -Q108 compared with cells expressing wild-type ATXN2-Q22. To control for equal transfection, we monitored levels of GFP, which was expressed as an independent cassette in the plasmid. Blots were re-probed for beta -actin as an internal loading control. The blot represents one of three independent experiments. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20153_rabbit-polyclonal-rgs8-antibody-310202416114723.jpg)
![Western Blot: RGS8 Antibody [NBP2-94343] - Analysis of extracts of various cell lines, using RGS8 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP2-94343-img0001.jpg "Western Blot: RGS8 Antibody [NBP2-94343] - Analysis of extracts of various cell lines, using RGS8 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.")
![Immunohistochemistry-Paraffin: RGS8 Antibody [NBP2-69025] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/RGS8-Antibody-Immunohistochemistry-Paraffin-NBP2-69025-img0001.jpg "Immunohistochemistry-Paraffin: RGS8 Antibody [NBP2-69025] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.")
![Western Blot: RGS8 Antibody [NBP1-80510] - HepG2 cell lysate, concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/RGS8-Antibody-Western-Blot-NBP1-80510-img0002.jpg "Western Blot: RGS8 Antibody [NBP1-80510] - HepG2 cell lysate, concentration 5.0ug/ml.")