RbBP5 Antibodies
We offer RbBP5 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each RbBP5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RbBP5 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the RbBP5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RbBP5 polyclonal antibodies.
Alternate Names for RbBP5 Antibodies
anti-RbBP5 antibody, anti-RBQ3RBBP-5 antibody, anti-retinoblastoma binding protein 5 antibody, anti-Retinoblastoma-binding protein RBQ-3 antibody, anti-Set1c WD40 repeat protein, homolog antibody, anti-SWD1 antibody
7 Results for "rbbp5" in Primary Antibodies
![Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NB600-252-img0012.jpg "Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-NB600-252-img0008.jpg)
![Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB600-252-img0009.jpg)
![Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NB600-252-img0011.jpg)
![Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-252 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Chromatin-Immunoprecipitation-NB600-252-img0007.jpg)
![Western Blot: RbBP5 Antibody [NBP2-95234] - Analysis of extracts of various cell lines, using RbBP5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NBP2-95234-img0005.jpg "Western Blot: RbBP5 Antibody [NBP2-95234] - Analysis of extracts of various cell lines, using RbBP5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: RbBP5 Antibody [NBP2-95234] - Analysis of A-549 cells using RbBP5 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95234-img0001.jpg)
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Rat Intestine using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-Paraffin-NBP2-95234-img0004.jpg)
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Human colon carcinoma using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-Paraffin-NBP2-95234-img0002.jpg)
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-95234] - Mouse liver using RBBP5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-Paraffin-NBP2-95234-img0003.jpg)
![Immunoprecipitation: RbBP5 Antibody [NBP2-95234] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug RbBP5 antibody (NBP2-95234). Western blot was performed from the immunoprecipitate using RbBP5 antibody (NBP2-95234) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunoprecipitation-NBP2-95234-img0006.jpg)
![Western Blot: RbBP5 Antibody [NBP2-34100] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NBP2-34100-img0004.jpg "Western Blot: RbBP5 Antibody [NBP2-34100] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG")
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NBP2-34100] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-Paraffin-NBP2-34100-img0005.jpg)
![Western Blot: RbBP5 Antibody [NBP2-55085] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NBP2-55085-img0002.jpg "Western Blot: RbBP5 Antibody [NBP2-55085] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: RbBP5 Antibody [NBP2-55085] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55085-img0001.jpg)
![Western Blot: RbBP5 Antibody (PSH0-15) [NBP3-32895] - Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (NBP3-32895) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32895) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32895_rabbit-rbbp5-mab-psh0-15-288202412461631.jpg "Western Blot: RbBP5 Antibody (PSH0-15) [NBP3-32895] - Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (NBP3-32895) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32895) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RbBP5 Antibody (PSH0-16) [NBP3-32896] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RbBP5 antibody (NBP3-32896) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32896_rabbit-rbbp5-mab-psh0-16-29820241411544.jpg "Immunohistochemistry: RbBP5 Antibody (PSH0-16) [NBP3-32896] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RbBP5 antibody (NBP3-32896) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: RbBP5 Antibody [H00005929-B01P] - Analysis of RBBP5 expression in transfected 293T cell line by RBBP5 polyclonal antibody. Lane 1: RBBP5 transfected lysate(59.18 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-H00005929-B01P-img0001.jpg "Western Blot: RbBP5 Antibody [H00005929-B01P] - Analysis of RBBP5 expression in transfected 293T cell line by RBBP5 polyclonal antibody. Lane 1: RBBP5 transfected lysate(59.18 KDa). Lane 2: Non-transfected lysate.")