Ran Antibodies
We offer Ran Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each Ran Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Ran Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Rat, Xenopus. Use the list below to choose the Ran Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Ran polyclonal antibodies and browse our Ran monoclonal antibody catalog.
Alternate Names for Ran Antibodies
anti-Ran antibody, anti-Androgen receptor-associated protein 24 antibody, anti-ARA24Gsp1 antibody, anti-GTP-binding nuclear protein Ran antibody, anti-GTPase Ran antibody, anti-guanosine triphosphatase Ran antibody, anti-member RAS oncogene family antibody, anti-OK/SW-cl.81 antibody, anti-RAN, member RAS oncogene family antibody, anti-RanGTPase antibody, anti-Ras-like protein TC4 antibody, anti-Ras-related nuclear protein antibody, anti-TC4 antibody
14 Results for "ran" in Primary Antibodies
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0009.jpg "Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0005.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0007.jpg)
![Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Flow-Cytometry-NBP2-61832-img0002.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0008.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0004.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0006.jpg)
![ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-ELISA-NBP2-61832-img0001.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - RanGTPase detected with Mouse Monoclonal Ran Antibody (8D1A6) in HEK293T and LNCaP C42. Lanes are labeled as follows: 1. Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4. LNCaP C42 5. LNCaP C42. Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with infrared scanner. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-61832_mouse-monoclonal-ran-antibody-8d1a6-western-blot-162023155342.jpg)
![Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-31776-img0004.jpg "Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-31776] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31776-img0002.jpg)
![Western Blot: Ran Antibody [NBP1-31776] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-31776-img0003.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0006.jpg "Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded rectum cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0004.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0005.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mouse mAb against A431 (1), C6 (2), Jurkat (3), Hela (4), COS7 (5), and Jurkat (6) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0007.jpg)
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded esophageal cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0003.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0001.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0002.jpg)
![Western Blot: Ran Antibody [NB100-1442] - Staining of A431 with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NB100-1442-img0001.jpg "Western Blot: Ran Antibody [NB100-1442] - Staining of A431 with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Ran Antibody [NB100-1442] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-Paraffin-NB100-1442-img0003.jpg)
![Immunohistochemistry-Paraffin: Ran Antibody [NB100-1442] - Staining of paraffin embedded Human Tonsil with antibody at 3 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-Paraffin-NB100-1442-img0002.jpg)
![Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0003.jpg "Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-68870] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-68870-img0005.jpg)
![Western Blot: Ran Antibody [NBP1-68870] - Staining of Human Testis lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP1-68870-img0002.jpg)
![Western Blot: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP2-32280-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Ran Antibody [NBP2-32280] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32280-img0004.jpg)
![Immunohistochemistry: Ran Antibody [NBP2-32280] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32280-img0003.jpg)
![Immunoprecipitation: Ran Antibody [NBP2-32280] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32280 used for IP at 6 ug per reaction. For blotting immunoprecipitated RAN, NBP2-32280 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunoprecipitation-NBP2-32280-img0002.jpg)
![Western Blot: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Western-Blot-NBP2-32281-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0004.jpg)
![Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0003.jpg)
![Immunoprecipitation: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for IP at 6 ug per reaction. For blotting immunoprecipitated RAN, NBP2-32281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Ran-Antibody-Immunoprecipitation-NBP2-32281-img0002.jpg)
![Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.](http://images.novusbio.com/fullsize/Ran-Antibody-ARAN1-Western-Blot-NB600-1082-img0001.jpg "Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.")
![Immunohistochemistry: Ran Antibody (15A8) [NBP3-26352] - Image of Ran Antibody (15A8) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-262202414471545.jpg "Immunohistochemistry: Ran Antibody (15A8) [NBP3-26352] - Image of Ran Antibody (15A8) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (15A8) [NBP3-26352] - Staining of Hela with Ran Antibody (15A8) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 527-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-28220241312242.jpg)
![Flow Cytometry: Ran Antibody (15A8) [NBP3-26352] - Overlay Peak curve showing Hela cells stained with Ran Antibody (15A8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26352_rabbit-ran-mab-15a8-282202414312723.jpg)
![Western Blot: Ran Antibody (SR1381) [NBP3-21795] - Western blot analysis using NBP3-21795 in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21795_rabbit-ran-mab-sr1381-5720231611215.jpg "Western Blot: Ran Antibody (SR1381) [NBP3-21795] - Western blot analysis using NBP3-21795 in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.")
![Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-288202412442969.jpg "Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: LoVo cell lysate (15 ug/Lane) Lane 5: Jurkat cell lysate (15 ug/Lane) Lane 6: MDA-MB-231 cell lysate (15 ug/Lane) Lane 7: MCF7 cell lysate (15 ug/Lane) Lane 8: RAW264.7 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: Rat thymus tissue lysate (30 ug/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Ran Antibody (JE33-28) [NBP3-32892] - Flow cytometric analysis of HeLa cells labeling Ran. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32892, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32892_rabbit-ran-mab-je33-28-392024884630.jpg)
![Western Blot: Ran Antibody (6S5X5) [NBP3-16393] - Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (NBP3-16393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Ran-Antibody-6S5X5-Western-Blot-NBP3-16393-img0001.jpg "Western Blot: Ran Antibody (6S5X5) [NBP3-16393] - Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (NBP3-16393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")