PIAS3 Antibodies
We offer PIAS3 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PIAS3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PIAS3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PIAS3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PIAS3 polyclonal antibodies and browse our PIAS3 monoclonal antibody catalog.
Alternate Names for PIAS3 Antibodies
anti-PIAS3 antibody, anti-MIZ-type containing 5 antibody, anti-Protein inhibitor of activated STAT protein 3 antibody, anti-protein inhibitor of activated STAT, 3 antibody
9 Results for "pias3" in Primary Antibodies
![Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Western-Blot-NBP1-77160-img0001.jpg "Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.")
![Western Blot: PIAS3 Antibody [NBP1-77160] - Western for PIAS3 in THP1 macrophage cells, Image from verified customer review.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Western-Blot-NBP1-77160-img0003.jpg)
![Immunohistochemistry: PIAS3 Antibody - BSA Free [NBP1-77160] - Immunohistochemistry of PIAS3 in human breast carcinoma tissue with PIAS3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77160_rabbit-polyclonal-pias3-antibody-2711202420175189.jpg)
![Western Blot: PIAS3 Antibody (JE30-36) [NBP3-32820] - Western blot analysis of PIAS3 on different lysates with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: HL-60 cell lysate Lane 3: Rat testis tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32820) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-288202412461661.jpg "Western Blot: PIAS3 Antibody (JE30-36) [NBP3-32820] - Western blot analysis of PIAS3 on different lysates with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: HL-60 cell lysate Lane 3: Rat testis tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32820) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PIAS3 Antibody (JE30-36) [NBP3-32820] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32820) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-298202414262620.jpg)
![Immunocytochemistry/ Immunofluorescence: PIAS3 Antibody (JE30-36) [NBP3-32820] - Immunocytochemistry analysis of NCI-H441 cells labeling PIAS3 with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-2920241192381.jpg)
![Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIAS3-Antibody-4F12-Western-Blot-H00010401-M03-img0003.jpg "Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi or non-transfected control. Blot probed with H00010401-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/PIAS3-Antibody-4F12-Western-Blot-H00010401-M03-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PIAS3 Antibody [NBP2-57238] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57238-img0001.jpg "Immunocytochemistry/Immunofluorescence: PIAS3 Antibody [NBP2-57238] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Immunohistochemistry: PIAS3 Antibody [NBP2-48503] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunohistochemistry-NBP2-48503-img0001.jpg "Immunohistochemistry: PIAS3 Antibody [NBP2-48503] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: PIAS3 Antibody [NBP2-98547] - Immunochemical staining of human PIAS3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunohistochemistry-Paraffin-NBP2-98547-img0001.jpg "Immunohistochemistry-Paraffin: PIAS3 Antibody [NBP2-98547] - Immunochemical staining of human PIAS3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")