PHGDH Antibodies
We offer PHGDH Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each PHGDH Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PHGDH Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PHGDH Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PHGDH polyclonal antibodies and browse our PHGDH monoclonal antibody catalog.
Alternate Names for PHGDH Antibodies
anti-PHGDH antibody, anti-3,3-phosphoglycerate dehydrogenase antibody, anti-3-PGDH antibody, anti-3PGDH antibody, anti-D-3-phosphoglycerate dehydrogenase antibody, anti-EC 1.1.1 antibody, anti-EC 1.1.1.95 antibody, anti-MGC3017 antibody, anti-PDG antibody, anti-PGAD antibody, anti-PGD antibody, anti-PGDH antibody, anti-phosphoglycerate dehydrogenase antibody, anti-SERA antibody
12 Results for "phgdh" in Primary Antibodies
![Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in human cell lines SK-MEL-30 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87311-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in human cell lines SK-MEL-30 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87311] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87311-img0016.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0013.jpg)
![Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87311-img0015.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0010.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0011.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0012.jpg)
![Simple Western: PHGDH Antibody [NBP1-87311] - Simple Western lane view shows a specific band for PHGDH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Simple-Western-NBP1-87311-img0007.jpg)
![Simple Western: PHGDH Antibody [NBP1-87311] - Electropherogram image(s) of corresponding Simple Western lane view. PHGDH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/PHGDH-Antibody-Simple-Western-NBP1-87311-img0008.jpg)
![Western Blot: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0014.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0008.jpg)
![Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0012.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0005.jpg)
![Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunoprecipitation-NBP1-32201-img0011.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0018.jpg "Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunocytochemistry-Immunofluorescence-H00026227-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunohistochemistry-Paraffin-H00026227-M01-img0014.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in A-431.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0016.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6 Analysis of PHGDH expression in Jurkat.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0017.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01), clone 4A3-1D6.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunohistochemistry-Paraffin-H00026227-M01-img0012.jpg)
![Immunoprecipitation: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunoprecipitation-H00026227-M01-img0015.jpg)
![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0008.jpg "Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] - Staining of MCF7 cells using the Anti-PHGDH monoclonal antibody, showing specific staining in the cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Immunocytochemistry-Immunofluorescence-NBP2-52939-img0009.jpg)
![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Analysis in human pancreas and skeletal muscle tissues using NBP2-52939 antibody. Corresponding PHGDH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393027.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393135..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393244..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393356..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-18820239356..jpg)
![Western Blot: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0001.jpg)
![Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801] - Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody [N3C2], Internal diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Frozen-NBP2-19801-img0004.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP2-19801] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP2-19801-img0002.jpg)
![Western Blot: PHGDH Antibody [NBP1-87310] - Analysis using Anti-PHGDH antibody NBP1-87310 (A) shows similar pattern to independent antibody NBP1-87311 (B).](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87310-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PHGDH Antibody [NBP1-87310] - Analysis using Anti-PHGDH antibody NBP1-87310 (A) shows similar pattern to independent antibody NBP1-87311 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87310] - Staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87310-img0007.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0013.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human cervix, uterine shows nuclear and cytoplasmic positivity in squamous cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0009.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0012.jpg)
![Western Blot: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.](http://images.novusbio.com/fullsize/PHGDH-Antibody-5C4-Western-Blot-NBP2-46389-img0002.jpg "Western Blot: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.")
![Immunohistochemistry: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/PHGDH-Antibody-5C4-Immunohistochemistry-NBP2-46389-img0001.jpg)
![Immunohistochemistry;PHGDH Antibody[NBP3-29511]Detection of human PHGDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHGDH used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29535_rabbit-phgdh-pab-78202410153528.jpg "Immunohistochemistry;PHGDH Antibody[NBP3-29511]Detection of human PHGDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHGDH used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human skin with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-136202422345037.png "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human skin with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP3-18850] - Immunofluorescence staining of PHGDH in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-18850 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-13620242234503.png)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human breast carcinoma with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-136202422345032.png)
![Western Blot: PHGDH Antibody (4D5-1F11) [H00026227-M02] - Detection against Immunogen (84.37 KDa) .](http://images.novusbio.com/fullsize/PHGDH-Antibody-4D5-1F11-Western-Blot-H00026227-M02-img0001.jpg "Western Blot: PHGDH Antibody (4D5-1F11) [H00026227-M02] - Detection against Immunogen (84.37 KDa) .")