NUP188 Antibodies
We offer NUP188 Antibodies for use in common research applications: Immunoprecipitation, Western Blot. Each NUP188 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NUP188 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the NUP188 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NUP188 polyclonal antibodies.
Alternate Names for NUP188 Antibodies
anti-NUP188 antibody, anti-FLJ21639 antibody, anti-KIAA0169hNup188 antibody, anti-nucleoporin 188kDa antibody, anti-nucleoporin NUP188 homolog antibody
7 Results for "nup188" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP2-38936] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38936-img0003.jpg "Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP2-38936] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: NUP188 Antibody [NBP2-38936] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunohistochemistry-Paraffin-NBP2-38936-img0007.jpg)
![Immunohistochemistry-Paraffin: NUP188 Antibody [NBP2-38936] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunohistochemistry-Paraffin-NBP2-38936-img0004.jpg)
![Immunohistochemistry-Paraffin: NUP188 Antibody [NBP2-38936] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunohistochemistry-Paraffin-NBP2-38936-img0005.jpg)
![Immunohistochemistry-Paraffin: NUP188 Antibody [NBP2-38936] - Staining of human rectum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunohistochemistry-Paraffin-NBP2-38936-img0006.jpg)
![Western Blot: NUP188 Antibody [NBP1-28748] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28748 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28717, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/NUP188-Antibody-Western-Blot-NBP1-28748-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NUP188 Antibody [NBP1-28748] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28748 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28717, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NUP188 Antibody [NBP1-28748] - Full induction of p21 is Nup155-dependent. a Protein fold-changes (blue & grey dots) including p53 targets (red dots) upon Nup155 (orange dot) knockdown compared to the control siRNA (AS) condition are shown as log2 ratios. p21 is highlighted in green as a key effector of the p53 response. Overall 3523 proteins could be quantified by quantitative mass spectrometry in HepG2 cells treated with Nutlin-3a for 24 h. b HepG2 cells were treated either with control siRNA (AS) or three different Nup155 siRNAs (Nup155#1, Nup155#2, & Nup155#3) for 72 h & p53 was induced by adding Nutlin-3a for 24 h. Cell extracts were analysed by immunoblotting with indicated antibodies (upper panel). Densitometric quantification of immunoblots derived from three independent experiments is shown in the lower panel & normalised to the control siRNA (AS) condition. c HepG2 cells were treated either with control siRNA (AS) or two different Nup35, Nup93, & Nup188 siRNAs (Nup35#1, Nup35#2, Nup93#1, Nup93#2, Nup188#1, & Nup188#2) for 72 h. Cells were harvested upon 24 h of Nutlin-3a treatment & extracts were analysed by immunoblotting with the indicated antibodies. For corresponding densitometric analyses see Supplementary Figure 1B-D. d Protein fold-changes of NPC components (purple dots) upon Nup155 knockdown (orange dot) corresponding to the conditions described in a. e Representative confocal microscopy pictures of MAb414 immunofluorescence staining of HepG2 cells treated as indicated. DAPI was used for DNA labeling. Scale bar = 10 µm. *p < 0.05, **p < 0.01, ***p < 0.001 (Student′s t-test). Data are presented as mean ± stdv. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31089132), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28748_rabbit-polyclonal-nup188-antibody-310202416163710.jpg)
![Western Blot: NUP188 Antibody [NBP1-28717] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28717 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28748, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/NUP188-Antibody-Western-Blot-NBP1-28717-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NUP188 Antibody [NBP1-28717] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28717 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28748, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP2-56516] - Staining of human cell line SiHa shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56516-img0001.jpg "Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP2-56516] - Staining of human cell line SiHa shows localization to nucleus & nucleoli.")
![Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP3-06066] - Immunofluorescence staining of NUP188 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUP188 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/NUP188-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06066-img0001.jpg "Immunocytochemistry/Immunofluorescence: NUP188 Antibody [NBP3-06066] - Immunofluorescence staining of NUP188 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUP188 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")