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![Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) labeling the ~125k monomer and the ~250k dimers for both mGluRs.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-5-1-Antibody-Western-Blot-NB300-126-img0002.jpg "Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) labeling the ~125k monomer and the ~250k dimers for both mGluRs.")
![Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5/1 protein using Mouse Anti-mGluR5/1 Monoclonal Antibody, Clone S75-33 (NBP2-22424). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5/1 Monoclonal Antibody (NBP2-22424) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Western-Blot-NBP2-22424-img0002.jpg "Western Blot: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5/1 protein using Mouse Anti-mGluR5/1 Monoclonal Antibody, Clone S75-33 (NBP2-22424). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5/1 Monoclonal Antibody (NBP2-22424) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.")
![Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-22424) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Immunocytochemistry-Immunofluorescence-NBP2-22424-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (S75-3) [NBP2-22424] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (NBP2-22424). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (NBP2-22424) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) mGluR1/5 glutamate receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/mGluR5-1-Antibody-S75-3-Immunocytochemistry-Immunofluorescence-NBP2-22424-img0004.jpg)