MELK Antibodies
We offer MELK Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each MELK Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MELK Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the MELK Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MELK polyclonal antibodies and browse our MELK monoclonal antibody catalog.
Alternate Names for MELK Antibodies
anti-MELK antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-hMELK antibody, anti-HPK38 antibody, anti-KIAA0175pEg3 kinase antibody, anti-maternal embryonic leucine zipper kinase antibody, anti-Protein kinase PK38 antibody
7 Results for "melk" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunocytochemistry-Immunofluorescence-NBP2-37481-img0002.jpg "Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunohistochemistry-NBP2-37481-img0005.jpg)
![Flow Cytometry: MELK Antibody (2G2) [NBP2-37481] - Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Flow-Cytometry-NBP2-37481-img0003.jpg)
![Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunohistochemistry-NBP2-37481-img0004.jpg)
![ELISA: MELK Antibody (2G2) [NBP2-37481] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-ELISA-NBP2-37481-img0001.jpg)
![Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0006.jpg "Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.")
![Western Blot: MELK Antibody [NB100-782] - NB100-782 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue NAC1 to Quaking > Goat Anti-PPP1R15A Antibody](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0001.jpg)
![Western Blot: MELK Antibody [NB100-782] - NB 100-782 (0.5ug/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0004.jpg)
![Western Blot: MELK Antibody (PSH03-55) [NBP3-32587] - Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (NBP3-32587) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32587) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32587_rabbit-melk-mab-psh03-55-27820241633126.jpg "Western Blot: MELK Antibody (PSH03-55) [NBP3-32587] - Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (NBP3-32587) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32587) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32587_rabbit-melk-mab-psh03-55-298202413585532.jpg)
![Western Blot: MELK Antibody [NBP1-83031] - Analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NBP1-83031-img0003.jpg "Western Blot: MELK Antibody [NBP1-83031] - Analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: MELK Antibody [NBP1-83031] - Staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MELK-Antibody-Immunohistochemistry-Paraffin-NBP1-83031-img0002.jpg)
![Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/MELK-Antibody-4F10X4-Western-Blot-NBP3-16211-img0001.jpg "Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western Blot: MELK Antibody (S06-4D6) [NBP3-15059] - Western blot detection of MELK in U2OS, C6 cell lysates using MELK Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 75kDa.](http://images.novusbio.com/fullsize/MELK-Antibody-S06-4D6-Western-Blot-NBP3-15059-img0001.jpg "Western Blot: MELK Antibody (S06-4D6) [NBP3-15059] - Western blot detection of MELK in U2OS, C6 cell lysates using MELK Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 75kDa.")