MCPIP1 Antibodies
MCPIP1 (Monocyte chemoattractant protein-induced protein 1), also called ZC3H12A and Regnase-1, is an endonuclease that functions as a negative regulator of inflammation. Human MCPIP1 protein has a theoretical molecular weight of 66 kDa and exhibits RNase activity to degrade many cytokine mRNA transcripts. We offer MCPIP1/ZC3H12A antibodies that have been featured in 5+ publications. Browse our catalog of monoclonal, polyclonal, and MCPIP1-conjugated antibodies. Validated research applications for our MCPIP1/ZC3H12A-antibodies include ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, and Western Blot. These MCPIP1 antibodies are reactive in Human and Mouse. View the technical details, images, references, reviews, and related products to help choose the anti- MCPIP1/ZC3H12A antibody best suited for your research needs. Each MCPIP1 antibody is fully covered by Novus’ Guarantee+.
Alternate Names for MCPIP1 Antibodies
anti-MCPIP1/ZC3H12A antibody, anti-ZC3H12A antibody, anti-zinc finger CCCH-type containing 12A antibody
8 Results for "mcpip1-zc3h12a" in Primary Antibodies
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0001.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - WB Validation in Human Lung. Loading: 15 ug of lysate per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 ug/mL Lane 2: 4 ug/mL")
![Western Blot: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation in Mouse Tissues.Loading: 15 ug of lysates per lane. Antibodies: ZC3H12A , 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419241348.gif)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: MCPIP1/ZC3H12A NBP3-18333, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-18333-img0003.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419381765.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419541514.jpg)
![Immunohistochemistry: MCPIP1/ZC3H12A Antibody - BSA Free [NBP3-18333] - Validation of ZC3H12A in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin](http://images.novusbio.com/fullsize/nbp3-18333_rabbit-mcpip1-zc3h12a-pab-2711202419475869.jpg)
![Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP3-18333] - Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling MCPIP1/ZC3H12A with NBP3-18333 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18333-img0004.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP2-37871-img0004.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human cell line U-251 MG shows localization to cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37871-img0002.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0008.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0005.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human colon shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0006.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP2-37871-img0007.jpg)
![Knockdown Validated: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249413347.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 | 20 | 20 | 10 | 10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249351328.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-2120249432887.jpg)
![Western Blot: MCPIP1/ZC3H12A Antibody (HL1442) - Azide and BSA Free [NBP3-25765] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (NBP3-25765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25765_rabbit-mcpip1-zc3h12a-mab-hl1442-azide-and-bsa-free-212024939128.jpg)
![Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP1-83587] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Immunohistochemistry-Paraffin-NBP1-83587-img0002.jpg "Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP1-83587] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.")
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP1-76258] - Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP1-76258-img0002.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP1-76258] - Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-03272] - Analysis of extracts of various cell lines, using MCPIP1/ZC3H12A antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/MCPIP1-ZC3H12A-Antibody-Western-Blot-NBP3-03272-img0001.jpg "Western Blot: MCPIP1/ZC3H12A Antibody [NBP3-03272] - Analysis of extracts of various cell lines, using MCPIP1/ZC3H12A antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")