mCherry Antibodies
mCherry is a monomeric red fluorescent protein (mRFP) with a theoretical molecular weight of 28 kDa belonging to the mFruits family. It is bright, photostable, and ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. We offer mCherry antibodies that have been cited in 100+ publications with multiple 4 and 5-star reviews. Browse our catalog of monoclonal antibodies, polyclonal antibodies, and mCherry-conjugated antibodies. Our mCherry antibodies have been validated for multiple research applications including ELISA, ICC, Immunofluorescence, IHC-Free Floating, IHC-Frozen, IHC-Paraffin, IHC-Whole Mount, Live Imaging Microscopy, Single-Cell Western, and Knockdown Validated Western Blot. These mCherry antibodies are suitable in multiple model species including Human, Mouse, Rat, Zebrafish, All Species, and Non-species Specific. Use the list below to choose the anti-mCherry antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews, and related products. Each mCherry antibody is fully covered by Novus’ Guarantee+.
Alternate Names for mCherry Antibodies
anti-mCherry antibody, anti-DSRED antibody, anti-red fluorescent protein mCherry antibody, anti-Red Fluoroscent Protein antibody
14 Results for "mcherry" in Primary Antibodies
![Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and Tg (isl1:cdh2_EC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with a-GFP (green) (A,D,G) and a-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg (isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg (isl1:GFP)/Tg(isl1:cdh2_EC-mCherry) vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0164433) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunohistochemistry-NBP1-96752-img0006.jpg "Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and Tg (isl1:cdh2_EC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with a-GFP (green) (A,D,G) and a-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg (isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg (isl1:GFP)/Tg(isl1:cdh2_EC-mCherry) vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0164433) licensed under a CC-BY license.")
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Analysis of HEK293 cell lysates and recombinant protein solutions using mCherry antibody, dilution 1:1,000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with GFP construct. Major band at about 30 kDa corresponds to mCherry protein (predicted molecular weight: 27 kDa). mCherry antibody does not react with GFP protein. The same blot was simultaneously probed with chicken HSP60 pAb, dilution 1:5,000 in red which reveals band at 60 kDa seen only in cell lysates.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0005.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 32-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from the western blots. GFP blots and WT worms serve as controls for loading and normalization respectively. Scale bar: 100 i1/4m. n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Nat Commun (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0008.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Threonine 276 is required for the intramolecular interaction, and for inhibition of membrane binding. Mutation of threonine 276 to aspartic acid promotes the association of cytohesin 2 with membranes. MDCK cells were transfected with constructs encoding mCherry-tagged wild-type or T276D cytohesin 2 and fractionated into cytosol and total membranes. The fractions were Western blotted with mouse anti-mCherry, mouse anti-E-cadherin and mouse anti-actin. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pone.0082084) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - HEK293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and NBP1-96752. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunocytochemistry-Immunofluorescence-NBP1-96752-img0003.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected HEK293 cells (lane -). NBP1-96752 binds a major band running at about 28 kDa (observed molecular weight) corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0002.jpg)
![Immunohistochemistry-Frozen: mCherry Antibody (1C51) [NBP1-96752] - Mouse Bone Marrow Sections (Femur). Fixed-frozen and decalcified. tdTomato reporter transgenic mice. tdTomato in hematopoietic cells were detected by anti-mCherry antibody. Antibody is cross-reactive and works well for fixed-frozen bone marrow. Background is low. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunohistochemistry-Frozen-NBP1-96752-img0004.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Mis18 alpha :Mis18 beta -hexamer mediates dimerization of M18BP1.(A) Analytical SEC results of M18BP11–140-MBP (cyan), M18BP11–228-MBP (red), Mis18 alpha :Mis18 beta :M18BP11–140-MBP (purple), Mis18 alpha :Mis18 beta :M18BP11–228-MBP (green). The elution volumes of thyroglobulin (670 kD), ferritin (440 kD), catalase (240 kD) & ovalbumin (44 kD) are shown as standards. Red lines indicate fractions collected for Tricine–SDS-PAGE analyses. Gels were stained with CBB. (B) Sedimentation velocity AUC results of the same samples used in the analytical SEC experiments (panel A). The best-fit size distributions are shown with the colors indicated in panel A. Data profiles used for curve-fitting analyses are shown in Figure 7—figure supplement 1. (C) Summary table of the results obtained from the AUC experiments of panel B. Sed. coef., sedimentation coefficient; MWobs., observed molecular weight; MWtheo., theoretical molecular weight. (D) Western blot results of co-immunoprecipitation experiments using GFP-Trap_A beads. HeLa CENP-A-SNAP + EGFP-M18BP11–140-P2A-T2A-mCherry-M18BP11–140, EGFP-M18BP11–140/T40D/S110E-P2A-T2A-mCherry-M18BP11–140/T40D/S110E, GST-EGFP-M18BP11–140-P2A-T2A-GST-mCherry-M18BP11–140, or GST-EGFP-M18BP11–140/T40D/S110E-P2A-T2A-GST-mCherry-M18BP11–140/T40D/S110E were analyzed.DOI://dx.doi.org/10.7554/eLife.23352.014Data profiles for AUC experiments.Best-fitting results of the sedimentation velocity AUC data of M18BP11–140-MBP, M18BP11–228-MBP, Mis18 alpha :Mis18 beta :M18BP11–140-MBP, and Mis18 alpha :Mis18 beta :M18BP11–228-MBP. Residuals represent the deviation of the continuous c(s) distribution model from the observed signals. The values of RMSD for data fitting are shown.DOI://dx.doi.org/10.7554/eLife.23352.015 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-96752_mouse-monoclonal-mcherry-antibody-1c51-310202416113527.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - The oligomerization state of overexpressed Cav1 varies as a function of its tag. COS-7 cells expressing the indicated constructs were lysed in digitonin & subjected to BN-PAGE followed by western blotting for Cav1 (red) & either GFP, mCherry or myc (green). A) Cells were either left untransfected (‘control’) or transfected with EGFP, Cav1-GFP or P132L-GFP. B) As in (A) except cells were transfected with the indicated mCherry constructs. C) As in (A) except cells were transfected with Cav1-myc or P132L-myc. Figures are representative of two independent experiments. Red arrows indicate the high molecular weight band positive for both tag antibodies & Cav1 antibodies (h1-97 or 2297). Black arrows indicate the high molecular weight band only positive for Cav1 antibodies (h1-97 or 2297). Green arrows indicate the low molecular weight bands only positive for FP tag antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25639341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-96752_mouse-monoclonal-mcherry-antibody-1c51-3102024161220.jpg)
![Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25157-img0011.jpg "Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.")
![Immunohistochemistry: mCherry Antibody [NBP2-25157] - Activation of TRPV1 receptors in ARC POMC neurons reduces food intake. Expression of Gi/o-DREADD in POMC neurons in the ARC (top left panel, scale bar: 20 um). Arrowheads represent POMC neurons that co-expressed mCherry (n = 493 out of 529 mCherry-positive neurons, n = 3 mice). Treatment with CNO (10 uM) hyperpolarized POMC neurons (top right panel; Control, -40.7 +/- 1.5 mV; CNO, -47.1 +/- 2.3 mV, n = 6 neurons, **p 50 measurements – 3 per cell; ***P < 0.001, one‐way ANOVA on ranks followed by Dunn's post hoc test). (C) Src‐transformed p130Cas−/−MEFs co‐expressing p130Cas (SC) & mouse Flag tagged PKN3 WT or Flag‐PKN3 mPR are shown. Cells were grown on FN‐coated coverslips for 48 h, fixed, & stained for p130Cas by anti‐pTyr165 p130Cas antibody (pY165 p130Cas; 2nd 405), for actin by Phalloidin 488 & for Flag‐PKN3 by anti‐Flag antibody (2nd 633). Reflection (670 nm) indicates fibronectin degradation. All scale bars represent 20 μm. Cell were imaged by Leica TCS SP8 microscope system equipped with Leica 63×/1.45 oil objective. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30422386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25157_rabbit-polyclonal-mcherry-antibody-310202416114713.jpg)
![Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in the adult retina. (A) Flat-mounted retina labeled with anti-mCherry antibody. (B,B’) mCherry (red) & Brn3 (teal) colocalization. Yellow arrow indicates a Brn3+ mCherry- cell. (C,C’) mCherry (red) & RBPMS (teal) colocalization. Yellow arrows indicate RBPMS+ mCherry- cell bodies. (D,D’) mCherry (red) & Tuj1 (teal) colocalization. White arrows indicate Tuj1+ mCherry+ axons. (E–E’’’) Cross-section of an adult retina labeled with mCherry (red), RBPMS (gray), DAPI (blue), & Pax6 (green). All mCherry+ cells (white arrows) are RBPMS+. Amacrine cells are labeled with yellow stars & are mCherry- (Pax6+ RBPMS− mCherry− cells). Yellow arrowhead corresponds to an mCherry− RGC (RBPMS+ Pax6− mCherry− cell). ONH: Optic Nerve Head. ONL: Outer Nuclear Layer. INL: Inner Nuclear Layer. GCL: Ganglion Cell Layer. Scale bars: 300 microns in A, 50 microns in B–E’’’. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31197108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25157_rabbit-polyclonal-mcherry-antibody-310202416114789.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0005.jpg "Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.")
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of NBP2-25158. Lane 1. Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector. There is a strong clean band at about 29kDa corresponding to mCherry (predicted molecular weight ~27kDa). Lane 2, lysate of untransfected HEK293 cells show no protein bands. The NBP2-25158 antibody was used at 1:2,000 dilution.](http://images.novusbio.com/fullsize/mCherry-Antibody-NBP2-25158-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of HEK293 cell lysates, and recombinant protein solutions using chicken mCherry pAb, dilution 1:2000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with an mCherry-HA constract, [4] pure recombinant mCherry protein (predicted molecular weight ~ 27kDa), [5] pure recombinant GFP protein and [6] HEK293 transfected with a GFP construct. The major band at about 30kDa corresponds to mCherry-HA protein and the slightly larger recombinant form runs at about 33kDa due to presence of a His tag and other vector derived sequence. The mCherry antibody reacts strongly with both mCherry constructs and does not react with GFP protein. The blot was simultaneously probed with mouse HSP60 mAb, dilution 1:10000 (Red), which reveals a band at 60kDa only in the cell lysates.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-25158-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - Dox-inducible DN Lats2-IRES-mCherry transgenic embryos were imaged before Dox treatment (top panel) and the same embryo was imaged following 24 hr of Dox live (middle panel) and fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, immunofluorescence stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos are circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 um. n indicates number of transgenic embryos analyzed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28226240/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0007.jpg)
![Western blot analysis of HEK293 cell lysates using chicken pAb to mCherry, NBP2-25158, dilution 1:2,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein molecular weight standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. NBP2-25158 recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.](http://images.novusbio.com/fullsize/nbp2-25158_chicken-polyclonal-mcherry-antibody-western-blot-264202414221..jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-9102024205672.jpg "Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-43720] - Analysis of Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min. Green: mCherry protein stained by mCherry antibody diluted at 1:500. Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43720-img0005.jpg)
![Immunohistochemistry Whole-Mount: mCherry Antibody [NBP2-43720] - Analysis of 2 days-post-fertilization zebrafish embryo. mCherry antibody labels mCherry-expressing cells dilution: 1:100.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunohistochemistry-Whole-Mount-NBP2-43720-img0001.jpg)
![Immunoprecipitation: mCherry Antibody [NBP2-43720] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunoprecipitation-NBP2-43720-img0002.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Analysis of A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12 % SDS-PAGE mCherry antibody dilution: 1:2000.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-43720-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-91020242014626.jpg)
![Western Blot: mCherry Antibody (235) [NBP2-43768] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [235] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-235-Western-Blot-NBP2-43768-img0001.jpg "Western Blot: mCherry Antibody (235) [NBP2-43768] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [235] dilution: 1:1000.")
![Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP3-05558-img0002.jpg "Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.")
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP3-05558] - Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with mCherry Antibody at a dilution of 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05558-img0001.jpg)
![Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-2120249342765.jpg "Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-2120249342713.jpg)
![Immunohistochemistry-Paraffin: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - mCherry antibody [HL1482] detects mCherry protein at cytoplasm by immunohistochemical analysis. Sample:Paraffin-embedded mock and mCherry tagged protein transfected 293T cell pellet. Green: mCherry stained by mCherry antibody [HL1482] (NBP3-25559) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-212024940869.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - mCherry antibody [HL1482] detects mCherry protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mCherry stained by mCherry antibody [HL1482] (NBP3-25559) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-2120249383172.jpg)
![Western Blot: mCherry Antibody [NBP2-43727] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-43727-img0006.jpg "Western Blot: mCherry Antibody [NBP2-43727] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: mCherry Antibody [NBP2-43727] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunoprecipitation-NBP2-43727-img0001.jpg)
![Western Blot: mCherry Antibody (857) [NBP2-43769] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [857] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-857-Western-Blot-NBP2-43769-img0001.jpg "Western Blot: mCherry Antibody (857) [NBP2-43769] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [857] dilution: 1:1000.")
![Immunocytochemistry/Immunofluorescence: mCherry Antibody (844) [NBP2-43778] - mCherry antibody detects mCherry protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mCherry stained by mCherry antibody diluted at 1:1000. Red: mCherry is expressed in the transfected cell. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/mCherry-Antibody-844-Immunocytochemistry-Immunofluorescence-NBP2-43778-img0002.jpg "Immunocytochemistry/Immunofluorescence: mCherry Antibody (844) [NBP2-43778] - mCherry antibody detects mCherry protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mCherry stained by mCherry antibody diluted at 1:1000. Red: mCherry is expressed in the transfected cell. Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Western Blot: mCherry Antibody (844) [NBP2-43778] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [844] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-844-Western-Blot-NBP2-43778-img0001.jpg)