MBD3 Antibodies
We offer MBD3 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each MBD3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MBD3 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the MBD3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MBD3 polyclonal antibodies and browse our MBD3 monoclonal antibody catalog.
Alternate Names for MBD3 Antibodies
anti-MBD3 antibody, anti-methyl-CpG binding domain protein 3 antibody, anti-methyl-CpG-binding domain protein 3 antibody, anti-Methyl-CpG-binding protein MBD3 antibody
8 Results for "mbd3" in Primary Antibodies
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0002.jpg "Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MBD3 Rabbit mAb (NBP3-16718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Immunohistochemistry-Paraffin-NBP3-16718-img0001.jpg)
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0003.jpg)
![Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/MBD3-Antibody-PCRP-MBD3-1C4-Flow-Cytometry-NBP3-07604-img0002.jpg "Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).")
![Protein Array: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-07604] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4).](http://images.novusbio.com/fullsize/MBD3-Antibody-PCRP-MBD3-1C4-Protein-Array-NBP3-07604-img0001.jpg)
![Western Blot: MBD3 Antibody [NBP3-18656] - Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with antibody against human MBD3 , diluted 1:2,000 in TBS-Tween with 5% skimmed milk. The MW marker (in kDa) is on the left; the location of the protein of interest is indicated on the right. Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Location of tagged MBD3 is indicated with an arrow.](http://images.novusbio.com/fullsize/MBD3-Antibody-Western-Blot-NBP3-18656-img0001.jpg "Western Blot: MBD3 Antibody [NBP3-18656] - Figure 2A: Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with antibody against human MBD3 , diluted 1:2,000 in TBS-Tween with 5% skimmed milk. The MW marker (in kDa) is on the left; the location of the protein of interest is indicated on the right. Figure 2B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An antibody directed against the TY1 tag or the antibody against MBD3 (1:2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Location of tagged MBD3 is indicated with an arrow.")
![ELISA: MBD3 Antibody [NBP3-18656] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD3 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,890.](http://images.novusbio.com/fullsize/MBD3 Antibody-ELISA-NBP3-18656-img0002.jpg)
![Western Blot: MBD3 Antibody [NBP3-04590] - Analysis of extracts of various cell lines, using MBD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic](http://images.novusbio.com/fullsize/MBD3-Antibody-Western-Blot-NBP3-04590-img0001.jpg "Western Blot: MBD3 Antibody [NBP3-04590] - Analysis of extracts of various cell lines, using MBD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic")
![Western Blot: MBD3 Antibody [NBP3-00136] - Anti-MBD3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/MBD3-Antibody-Western-Blot-NBP3-00136-img0002.jpg "Western Blot: MBD3 Antibody [NBP3-00136] - Anti-MBD3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).")