JNK1 Antibodies
We offer JNK1 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each JNK1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our JNK1 Antibodies can be used in a variety of model species: Bovine, C. elegans, Chicken, Human, Mouse, Rat. Use the list below to choose the JNK1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our JNK1 polyclonal antibodies and browse our JNK1 monoclonal antibody catalog.
Alternate Names for JNK1 Antibodies
anti-JNK1 antibody, anti-MAPK8 antibody, anti-c-Jun N-terminal kinase 1 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.24 antibody, anti-JNK-46 antibody, anti-JNK1 alpha protein kinase antibody, anti-JNK1 beta protein kinase antibody, anti-JNK1JNK1A2 antibody, anti-JUN N-terminal kinase antibody, anti-MAP kinase 8 antibody, anti-MAPK 8 antibody, anti-mitogen-activated protein kinase 8 antibody, anti-mitogen-activated protein kinase 8 isoform JNK1 alpha1 antibody, anti-mitogen-activated protein kinase 8 isoform JNK1 beta2 antibody, anti-PRKM8JNK antibody, anti-protein kinase JNK1 antibody, anti-SAPK1JNK21B1/2 antibody, anti-Stress-activated protein kinase 1 antibody, anti-Stress-activated protein kinase JNK1 antibody
12 Results for "jnk1" in Primary Antibodies
![Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Western-Blot-NBP1-48284-img0001.jpg "Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0008.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] - Immunofluorescent staining of HeLa cells using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48284-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Kidney tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human liver tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0005.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human Ovary tissue using anti-JNK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48284-img0006.jpg)
![Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.](http://images.novusbio.com/fullsize/JNK1-Antibody-Western-Blot-NB100-1925-img0004.jpg "Western Blot: JNK1 Antibody [NB100-1925] - Detection of JNK-1 using NB100-1925.")
![Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Western-Blot-H00005599-M11-img0010.jpg "Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of monoclonal antibody to MAPK8 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00005599-M11-img0005.jpg)
![Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi or non-transfected control. Blot probed with H00005599-M11. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Western-Blot-H00005599-M11-img0002.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between CRK and MAPK8. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0006.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between PXN and MAPK8. Huh7 cells were stained with anti-PXN rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0007.jpg)
![Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between BAD and MAPK8. Mahlavu cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Proximity-Ligation-Assay-H00005599-M11-img0008.jpg)
![Sandwich ELISA: JNK1 Antibody (2F11) [H00005599-M11] - Detection limit for recombinant GST tagged MAPK8 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/JNK1-Antibody-2F11-Sandwich-ELISA-H00005599-M11-img0009.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0004.jpg "Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.")
![Flow Cytometry: JNK1 Antibody (1E5) [NBP2-22209] - Flow cytometric analysis of HeLa cells using JNK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Flow-Cytometry-NBP2-22209-img0001.jpg)
![Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using MAPK8 mAb against human JNK1 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-Western-Blot-NBP2-22209-img0003.jpg)
![ELISA: JNK1 Antibody (1E5) [NBP2-22209] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/JNK1-Antibody-1E5-ELISA-NBP2-22209-img0002.jpg)
![Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Western-Blot-NBP3-16536-img0005.jpg "Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0004.jpg)
![Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunohistochemistry-Paraffin-NBP3-16536-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of NIH-3T3 cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-Antibody-8Q7S4-Immunocytochemistry-Immunofluorescence-NBP3-16536-img0003.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Immunohistochemistry-Paraffin-NBP3-13678-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/JNK1-p-Thr183-p-Tyr185-Antibody-HL1008-Western-Blot-NBP3-13678-img0001.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-9102024204127.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020242013406.jpg)
![Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) PC-12 whole cell extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020241959351.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0001.jpg "Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells using NB100-82009 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).")
![Immunohistochemistry: JNK1 [p Thr183] Antibody [NB100-82009] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (phospho-Thr183) antibody.](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Immunohistochemistry-NB100-82009-img0002.jpg)
![Western Blot: JNK1 [p Thr183] Antibody [NB100-82009] - Analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody](http://images.novusbio.com/fullsize/JNK1-[p-Thr183]-Antibody-Western-Blot-NB100-82009-img0003.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)
![Western Blot: JNK1 Antibody (S05-7A3) [NBP3-19929] - Western blot detection of JNK1 in Jurkat, Rat Brain, C6 cell lysates using NBP3-19929 (1:1000 diluted). Predicted band size: 48kDa. Observed band size: 46kDa.](http://images.novusbio.com/fullsize/JNK1-Antibody-S05-7A3-Western-Blot-NBP3-19929-img0001.jpg "Western Blot: JNK1 Antibody (S05-7A3) [NBP3-19929] - Western blot detection of JNK1 in Jurkat, Rat Brain, C6 cell lysates using NBP3-19929 (1:1000 diluted). Predicted band size: 48kDa. Observed band size: 46kDa.")