Jumonji/JARID2 Antibodies
We offer Jumonji/JARID2 Antibodies for use in common research applications: Chromatin Immunoprecipitation, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each Jumonji/JARID2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Jumonji/JARID2 Antibodies can be used in a variety of model species: Human, Mouse, Rabbit. Use the list below to choose the Jumonji/JARID2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Jumonji/JARID2 polyclonal antibodies and browse our Jumonji/JARID2 monoclonal antibody catalog.
Alternate Names for Jumonji/JARID2 Antibodies
anti-Jumonji/JARID2 antibody, anti-JARID2 antibody, anti-JMJjumonji-like protein antibody, anti-jumonji (mouse) homolog antibody, anti-jumonji homolog antibody, anti-jumonji, AT rich interactive domain 2 antibody, anti-Jumonji/ARID domain-containing protein 2 antibody, anti-protein Jumonji antibody
10 Results for "jumonji-jarid2" in Primary Antibodies
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13661) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Knockout-Validated-NB100-2214-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13661) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2214-img0001.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NB100-2214-img0008.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NB100-2214-img0006.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NB100-2214-img0005.jpg)
![Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Simple-Western-NB100-2214-img0007.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes(a) Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) & pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or JARID2 KO mESCs (right). Full blots are shown in Supplementary Fig. 9. (b) JARID2 1–530 binds to H2AK119u1-modified mononucleosomes (yellow) more tightly than unmodified (blue) or H2AK199u1(I44A) (red) as monitored by biolayer interferometry (n=3). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27892467), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2214_rabbit-polyclonal-jumonji-jarid2-antibody-310202415392539.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NBP3-00588-img0003.jpg "Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP3-00588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Jumonji/JARID2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00588-img0002.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NBP3-00588] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Jumonji / JARID2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Jumonji / JARID2 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NBP3-00588-img0001.jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)
![Western Blot: Jumonji/JARID2 Antibody (3L5C5) [NBP3-16839] - Western blot analysis of extracts of various cell lines, using Jumonji/JARID2 Rabbit mAb (NBP3-16839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-3L5C5-Western-Blot-NBP3-16839-img0001.jpg "Western Blot: Jumonji/JARID2 Antibody (3L5C5) [NBP3-16839] - Western blot analysis of extracts of various cell lines, using Jumonji/JARID2 Rabbit mAb (NBP3-16839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Jumonji/JARID2 Antibody (3L5C5) [NBP3-16839] - Western blot analysis of extracts of various cell lines, using Jumonji/JARID2 Rabbit mAb (NBP3-16839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-3L5C5-Western-Blot-NBP3-16839-img0002.jpg)
![Western Blot: Jumonji/JARID2 Antibody (S06-1K4) [NBP3-20079] - Western blot detection of Jumonji/JARID2 in K562, C6 cell lysates using NBP3-20079 (1:1000 diluted). Predicted band size: 139kDa. Observed band size: 139kDa.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-S06-1K4-Western-Blot-NBP3-20079-img0001.jpg "Western Blot: Jumonji/JARID2 Antibody (S06-1K4) [NBP3-20079] - Western blot detection of Jumonji/JARID2 in K562, C6 cell lysates using NBP3-20079 (1:1000 diluted). Predicted band size: 139kDa. Observed band size: 139kDa.")
![Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP2-57019] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57019-img0001.jpg "Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP2-57019] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & mitochondria.")