GRB7 Antibodies
We offer GRB7 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each GRB7 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GRB7 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the GRB7 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GRB7 polyclonal antibodies and browse our GRB7 monoclonal antibody catalog.
Alternate Names for GRB7 Antibodies
anti-GRB7 antibody, anti-B47 antibody, anti-Epidermal growth factor receptor GRB-7 antibody, anti-GRB7 adapter protein antibody, anti-growth factor receptor-bound protein 7 antibody
8 Results for "grb7" in Primary Antibodies
![Western Blot: GRB7 Antibody (JE50-05) [NBP3-32404] - Western blot analysis of GRB7 on different lysates with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/1,000 dilution. Lane 1: A431 cell lysate (40 ug/Lane) Lane 2: HepG2 cell lysate (40 ug/Lane) Lane 3: SK-Br-3 cell lysate (20 ug/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-27820241521241.jpg "Western Blot: GRB7 Antibody (JE50-05) [NBP3-32404] - Western blot analysis of GRB7 on different lysates with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/1,000 dilution. Lane 1: A431 cell lysate (40 ug/Lane) Lane 2: HepG2 cell lysate (40 ug/Lane) Lane 3: SK-Br-3 cell lysate (20 ug/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRB7 Antibody (JE50-05) [NBP3-32404] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32404) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29820241420977.jpg)
![Immunocytochemistry/ Immunofluorescence: GRB7 Antibody (JE50-05) [NBP3-32404] - Immunocytochemistry analysis of SK-Br-3 cells labeling GRB7 with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29202410484861.jpg)
![Flow Cytometry: GRB7 Antibody (JE50-05) [NBP3-32404] - Flow cytometric analysis of SK-Br-3 cells labeling GRB7. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32404, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29202416453299.jpg)
![Western Blot: GRB7 Antibody (SR2057) [NBP3-21932] - Western blot analysis using NBP3-21932 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21932_rabbit-grb7-mab-sr2057-672023106278.jpg "Western Blot: GRB7 Antibody (SR2057) [NBP3-21932] - Western blot analysis using NBP3-21932 in A431 cell lysate.")
![Western Blot: GRB7 Antibody [NB100-1027] - Antibody at 1ug/ml. Staining of A431 lysate (35u g protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GRB7-Antibody-Western-Blot-NB100-1027-img0002.jpg "Western Blot: GRB7 Antibody [NB100-1027] - Antibody at 1ug/ml. Staining of A431 lysate (35u g protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: GRB7 Antibody (3C12) [H00002886-M03] - Analysis of GRB7 expression in transfected 293T cell line by GRB7 monoclonal antibody (M03), clone 3C12. Lane 1: GRB7 transfected lysatE (59.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GRB7-Antibody-3C12-Western-Blot-H00002886-M03-img0002.jpg "Western Blot: GRB7 Antibody (3C12) [H00002886-M03] - Analysis of GRB7 expression in transfected 293T cell line by GRB7 monoclonal antibody (M03), clone 3C12. Lane 1: GRB7 transfected lysatE (59.7 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: GRB7 Antibody (3C12) [H00002886-M03] - Analysis of GRB7 transfected lysate using anti-GRB7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GRB7 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/GRB7-Antibody-3C12-Immunoprecipitation-H00002886-M03-img0001.jpg)
![Immunohistochemistry: GRB7 Antibody [NBP2-49243] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/GRB7-Antibody-Immunohistochemistry-NBP2-49243-img0001.jpg "Immunohistochemistry: GRB7 Antibody [NBP2-49243] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Western Blot: GRB7 Antibody [NBP1-30964] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE GRB7 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GRB7-Antibody-Western-Blot-NBP1-30964-img0002.jpg "Western Blot: GRB7 Antibody [NBP1-30964] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE GRB7 antibody, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: GRB7 Antibody [NBP3-14366] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/GRB7 Antibody-Immunohistochemistry-Paraffin-NBP3-14366-img0001.jpg "Immunohistochemistry-Paraffin: GRB7 Antibody [NBP3-14366] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)")