GRB2 Antibodies
We offer GRB2 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Peptide ELISA, Western Blot. Each GRB2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GRB2 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the GRB2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GRB2 polyclonal antibodies and browse our GRB2 monoclonal antibody catalog.
Alternate Names for GRB2 Antibodies
anti-GRB2 antibody, anti-abundant SRC homology antibody, anti-Adapter protein GRB2 antibody, anti-ASH antibody, anti-EGFRBP-GRB2 antibody, anti-epidermal growth factor receptor-binding protein GRB2 antibody, anti-Grb3-3 antibody, anti-growth factor receptor-bound protein 2 antibody, anti-growth factor receptor-bound protein 3 antibody, anti-HT027 antibody, anti-MST084 antibody, anti-MSTP084 antibody, anti-NCKAP2 antibody, anti-Protein Ash antibody, anti-SH2/SH3 adapter GRB2 antibody
12 Results for "grb2" in Primary Antibodies
![Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0012.jpg "Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunohistochemistry-Paraffin-NB100-866-img0014.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0010.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0011.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - (1ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunohistochemistry-Paraffin-NB100-866-img0013.jpg)
![Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Western-Blot-NBP3-15638-img0005.jpg "Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: GRB2 Antibody (9K6H0) [NBP3-15638] - Immunofluorescence analysis of HeLa cells using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunocytochemistry-Immunofluorescence-NBP3-15638-img0004.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Mouse spleen using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0003.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Rat spleen using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0001.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Human lung cancer using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0002.jpg)
![Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of 300ug extracts of 293T cells using 3ug GRB2 antibody (NBP3-15638). Western blot was performed from the immunoprecipitate using GRB2 (NBP3-15638) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunoprecipitation-NBP3-15638-img0006.jpg)
![Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-278202415295585.jpg "Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403] - Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202410334095.jpg)
![Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403] - Flow cytometric analysis of MCF7 cells labeling GRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32403, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202416372159.jpg)
![Western Blot: GRB2 Antibody (6E1) [NBP3-15167] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: GRB2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 21 KDa. Observed band size: 28 KDa.](http://images.novusbio.com/fullsize/GRB2-Antibody-6E1-Western-Blot-NBP3-15167-img0002.jpg "Western Blot: GRB2 Antibody (6E1) [NBP3-15167] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: GRB2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 21 KDa. Observed band size: 28 KDa.")
![Immunohistochemistry: GRB2 Antibody (6E1) [NBP3-15167] - IHC image of GRB2 antibody diluted at 1:100 and staining in paraffin-embedded human ovarian cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/GRB2-Antibody-6E1-Immunohistochemistry-NBP3-15167-img0001.jpg)
![Western Blot: GRB2 Antibody (SR1396) [NBP3-21851] - Western blot analysis using NBP3-21851 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21851_rabbit-grb2-mab-sr1396-57202315545124.jpg "Western Blot: GRB2 Antibody (SR1396) [NBP3-21851] - Western blot analysis using NBP3-21851 in HEK293 cell lysate.")
![Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NBP2-55208-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GRB2 Antibody [NBP2-55208] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55208-img0001.jpg)
![Western Blot: GRB2 Antibody [NBP2-55209] - Analysis using Anti-GRB2 antibody NBP2-55209 (A) shows similar pattern to independent antibody NBP2-55208 (B).](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NBP2-55209-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GRB2 Antibody [NBP2-55209] - Analysis using Anti-GRB2 antibody NBP2-55209 (A) shows similar pattern to independent antibody NBP2-55208 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GRB2 Antibody [NBP2-55209] - Staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55209-img0001.jpg)