GIT1 Antibodies
We offer GIT1 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each GIT1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GIT1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the GIT1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GIT1 polyclonal antibodies and browse our GIT1 monoclonal antibody catalog.
Alternate Names for GIT1 Antibodies
anti-GIT1 antibody, anti-ARF GAP GIT1 antibody, anti-ARF GTPase-activating protein GIT1 antibody, anti-CAT-1 antibody, anti-CAT1 antibody, anti-Cool-associated and tyrosine-phosphorylated protein 1 antibody, anti-G protein-coupled receptor kinase interacting ArfGAP 1 antibody, anti-G protein-coupled receptor kinase interactor 1 antibody, anti-G protein-coupled receptor kinase-interactor 1 antibody, anti-GRK-interacting protein 1 antibody
10 Results for "git1" in Primary Antibodies
![Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Western-Blot-NBP2-22423-img0003.jpg "Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0005.jpg)
![Western Blot: GIT1 Antibody [NBP1-31447] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-31447-img0008.jpg "Western Blot: GIT1 Antibody [NBP1-31447] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-31447] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31447-img0003.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31447-img0004.jpg)
![Western Blot: GIT1 Antibody [NBP1-31447] - Sample(30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-31447-img0005.jpg)
![Western Blot: GIT1 Antibody [NBP1-31447] - Rat2 whole cell lysate/extract.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-31447-img0006.jpg)
![Western Blot: GIT1 Antibody [NBP1-31447] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-31447-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-86144] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86144-img0006.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-86144] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.")
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0009.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0002.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human kidney shows strong cytoplasmic positivity in glomeruli.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0007.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-86144] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-86144-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03734-img0003.jpg "Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![ICC/IF-GIT1 Antibody [NBP3-03734] - Analysis of L929 cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GIT1-Antibody-Binding-Activity-NBP3-03734-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734] - Immunofluorescence analysis of C6 cells using [KO Validated] GIT1 Rabbit pAb (A15437) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03734_rabbit-polyclonal-git1-antibody-52202519313438.jpg)
![Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts of various cell lines, using GIT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GIT1-Antibody-Knockout-Validated-NBP3-03734-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/GIT1-Antibody-Knockout-Validated-NBP3-03734-img0004.jpg)
![Western Blot: GIT1 Antibody [NBP1-85798] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-85798-img0008.jpg "Western Blot: GIT1 Antibody [NBP1-85798] - Analysis in human cell line SCLC-21H.")
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-85798] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85798-img0006.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-85798-img0007.jpg)
![Western Blot: GIT1 Antibody [NBP1-85798] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-85798-img0005.jpg)
![Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/GIT1-Antibody-Western-Blot-NBP1-19141-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - Human breast carcinoma. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-19141-img0003.jpg)
![Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/GIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-19141-img0001.jpg)
