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FGF-8 Antibodies

We offer FGF-8 Antibodies for use in common research applications: Blocking/Neutralizing, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each FGF-8 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FGF-8 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the FGF-8 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FGF-8 polyclonal antibodies and browse our FGF-8 monoclonal antibody catalog.

Alternate Names for FGF-8 Antibodies

anti-FGF-8 antibody, anti-FGF8 antibody, anti-AIGFKAL6 antibody, anti-Androgen-induced growth factor antibody, anti-fibroblast growth factor 8 (androgen-induced) antibody, anti-fibroblast growth factor 8 antibody, anti-HBGF-8 antibody, anti-Heparin-binding growth factor 8 antibody, anti-MGC149376 antibody

10 Results for "fgf-8" in Primary Antibodies

MAB323

FGF-8 Antibody (47109)

(9 Publications)

100 μg

MAB323

Recombinant Mouse FGF-8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF-8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323). The ND50 is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).

FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Bafilomycin A1 (BAF) treatment demonstrates the polarization of ERK activity by FGF8 signal activity along the neuraltube. A-C) BAF amplifies ectopic ERK1/2 activity-related FGF8 induction revealing a clear polarized distribution of ERK1/2 activity around FGF8soaked beads depending of the implanted bead; rostral to IsO (C,D,G), or caudal to IsO (C,E). Nonetheles, isthmic organizer morphogenetic activityseems unaffected for Fgf8 (A) and negative regulator Sprouty 2 (B) expressions. Note that PBS bead implantation in control side (blue asterisk in C andF) did not show any ectopic induction. Two hours after bead implantation a clear amplified and almost non-homogeneous ERK1/2 activity wasdetected rostrally in the mesencephalon (rostral to the IsO), which was detected caudally when bead was placed in hindbrain (caudal to the IsO)territories (E). In telencephalic vesicles, caudal to the anr (H) the polarity of ERK activation was reversed. This polarized dpERK detection around thebead is lost at the zli (zona limitans intrathalamica) region (I). Similar symmetric ERK-related FGF8 signal found in zli was seen when placing a FGF8bead in the midbrain of Fgf8 hypomorphic mice (J,K). Importantly FGF8b protein distribution (M) was observed apparently in equal intensity andrange at rostral (N) and caudal (O) sides of the bead (for comparison with PBS bead in panel L). Scale bars are 0,5 mm in A, B, C, H, I, 200 mm in D, E, J,100 mm in F, G, K, L, M, and 50 mm in N, O. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC3391221/), licensed under a CC-BY license. Not internally tested by R&D Systems.

Phosphorylation patterns of ERK1/2 (dpERK) enzymes in the anterior neural tube.Whole mount in situ hybridization (ISH) of E9.5 mouse embryo (A,B,B’) and corresponding organotypic neural tissue cultures of mouse E9.5 anterior neural tube (A’,B”,C-E”; ONTCs; [42]) where it shows the maintenance of gene expression profiles such us Fgf8, Tcf4 (A,A’) (used to delimit main brain subdivisions such the diencephalon (D; Tcf4 positive) and the mesencephalic anlage (M; negative staining), Pax6 D-D” (delimiting di-mesencephalic boundary and rhombomere 1–2 limits) and En2 E-E”. In B-B”) are photomicrographs of E9.5 mouse embryo with anti-dpERK Immunohistochemistry (IHC) taken from lateral (B) and caudal (B’) sides and the corresponding IHC in ONTCs. (C) double staining procedure: ISH (in blue) for Fgf8 and IHC for dpERK (dark brown) to localize inside the dpERK domain the position of the IsO, marked by the solid red line. (D-E”) photomicrographs of same ONTCs in which first a whole mount ISH for Pax6 (D) or Tcf4/En2 (E) were made and afterwards IHC against dpERK. (D’,E’ respectively). Dashed lines mark the main transversal (in black) and longitudinal (in red) brain subdivisions. These ONTCs were cut into transversal sections to the isthmic constriction (D”,E”) to proof that indeed dpERK expression reaches diencephalic anlage (D”; see asterisk; rostral is left) and has a wider expansion than En2 expression (E”; see asterisk; rostral is left). anr is anterior neural ridge secondary organizer; ba is branchial arch; IsO is isthmic organizer; os is optic stalk; ov is otic vesicle; r is rhombomere; T is telencephalon, D, diencephalon, M, mesencephalon. Scale bars are 0,5 mm except in D”, E” they are 100 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22792203), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB323-SP
Carrier Free	MAB323-500

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AF-423-NA

FGF-8 Antibody [Unconjugated] - b Isoform

(3 Publications)

100 μg

AF-423-NA

Recombinant Mouse FGF‑8b Isoform (Catalog # 423-F8) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse FGF‑8b Isoform (60 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse FGF‑8b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA). The ND50 is typically 0.4-2.4 µg/mL in the presence of heparin (1 µg/mL).

FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Mouse FGF-8 b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF423

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-423-SP

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H00002253-M01

FGF-8 Antibody (2A10)

0.1 mg

H00002253-M01

Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in NIH/3T3.

Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in PC-12.

Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10 Analysis of FGF8 expression in HepG2.

Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Raw 264.7.

Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Jurkat.

Sandwich ELISA: FGF-8 Antibody (2A10) [H00002253-M01] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002253-M05

FGF-8 Antibody (2A11)

0.1 mg

H00002253-M05

ELISA: FGF-8 Antibody (2A11) [H00002253-M05] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Proximity Ligation Assay: FGF-8 Antibody (2A11) [H00002253-M05] - Analysis of protein-protein interactions between FGFR2 and FGF8. HeLa cells were stained with anti-FGFR2 rabbit purified polyclonal 1:1200 and anti-FGF8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c

Sandwich ELISA: FGF-8 Antibody (2A11) [H00002253-M05] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-98443

FGF-8 Antibody

100 ul

NBP1-98443

Western Blot: FGF-8 Antibody [NBP1-98443] - Host: Mouse. Target Name: FGF8. Sample Tissue: Mouse Pancreas. Antibody Dilution: 1ug/ml

Western Blot: FGF-8 Antibody [NBP1-98443] - PANC1 Cell Lysate 1.0ug/ml, Gel Concentration: 12%

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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H00002253-M06

FGF-8 Antibody (3H2)

0.1 mg

H00002253-M06

Western Blot: FGF-8 Antibody (3H2) [H00002253-M06] - Analysis of FGF8 expression in HepG2 (Cat # L019V1).

Sandwich ELISA: FGF-8 Antibody (3H2) [H00002253-M06] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002253-M04

FGF-8 Antibody (3B10)

0.1 mg

H00002253-M04

Sandwich ELISA: FGF-8 Antibody (3B10) [H00002253-M04] - Detection limit for recombinant GST tagged FGF8 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002253-M03

FGF-8 Antibody (2A12)

0.1 mg

H00002253-M03

ELISA: FGF-8 Antibody (2A12) [H00002253-M03] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-90327

FGF-8 Antibody (001)

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90327AF350
Alexa Fluor 405	NBP2-90327AF405
Alexa Fluor 488	NBP2-90327AF488
Alexa Fluor 532	NBP2-90327AF532
Alexa Fluor 594	NBP2-90327AF594
Alexa Fluor 647	NBP2-90327AF647
Alexa Fluor 700	NBP2-90327AF700
Alexa Fluor 750	NBP2-90327AF750
Allophycocyanin	NBP2-90327APC
Biotin	NBP2-90327B
CoraFluor 1	NBP2-90327CL1
DyLight 350	NBP2-90327UV
DyLight 405	NBP2-90327V
DyLight 488	NBP2-90327G
DyLight 550	NBP2-90327R
DyLight 594	NBP2-90327DL594
DyLight 650	NBP2-90327C
DyLight 680	NBP2-90327FR
DyLight 755	NBP2-90327IR
FITC	NBP2-90327F
HRP	NBP2-90327H
Janelia Fluor 525	NBP2-90327JF525
Janelia Fluor 549	NBP2-90327JF549
Janelia Fluor 585	NBP2-90327JF585
Janelia Fluor 635	NBP2-90327JF635
Janelia Fluor 646	NBP2-90327JF646
Janelia Fluor 669	NBP2-90327JF669
PE	NBP2-90327PE
PerCP	NBP2-90327PCP
mFluor Violet 450 SE	NBP2-90327MFV450
mFluor Violet 500 SE	NBP2-90327MFV500
mFluor Violet 610 SE	NBP2-90327MFV610

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BAF423

FGF-8 Antibody [Biotin]

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-423-NA
Carrier Free	AF-423-SP

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