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152 Results for "egf r erbb1"
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AF231

EGFR Antibody [Unconjugated]

(30 Publications)
100 μg
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, Flow, IHC, IP, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ICC/IF
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF231
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF231-SP
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AF1095

EGFR [p Tyr1173] Antibody [Unconjugated]

(10 Publications)
50 μg
AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1173) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1095-SP
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AF1280

EGFR Antibody [Unconjugated]

(1 Review)
(10 Publications)
100 μg
AF1280
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EGFR at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mu
Species Glossary
Reactivity: Mu
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF1280
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1280-SP
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DYC1095B-2

Human Phospho-EGFR DuoSet IC ELISA, 2 Plate

(14 Publications)
1 Kit (for 2 Plates)
DYC1095B-2
The human epidermoid carcinoma cell line, A431, was treated with 25 ng/mL recombinant human EGF (R&D Systems Catalog #236-EG) for 5 minutes to induce tyrosine phosphorylation of EGF R. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-EGF R monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with an HRP-conjugated anti-phospho-tyrosine monoclonal antibody (R&D Systems, Catalog #HAM1676) to detect phosphor-EGF R. Bands were visualized by chemiluminescent detection. Human Phospho-EGF R can be detected in this DuoSet IC ELISA by using approximately 2-4 times less lysate than is needed for a conventional IP-Western blot.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1095BE
Biotin DYC1095B-5
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free DYC1095BE
Carrier Free DYC1095B-5
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MAB3570

EGFR [p Tyr1068] Antibody (338324) [Unconjugated]

(1 Review)
(9 Publications)
100 μg
MAB3570
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, CyTOF-ready, Flow
Applications: WB, CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC3570U-100UG
Alexa Fluor 405 IC3570V-100UG
Alexa Fluor 488 IC3570G-100UG
Alexa Fluor 532 FAB3570X
Alexa Fluor 594 IC3570T-100UG
Alexa Fluor 647 IC3570R-100UG
Alexa Fluor 700 IC3570N-100UG
Alexa Fluor 750 IC3570S-100UG
Allophycocyanin FAB3570A
Biotin FAB3570B
CoraFluor 1 FAB3570CL1
DyLight 350 FAB3570D
DyLight 405 FAB3570E
DyLight 488 FAB3570K
DyLight 550 FAB3570L
DyLight 594 FAB3570M
DyLight 650 FAB3570W
DyLight 680 FAB3570Y
DyLight 755 FAB3570Z
Fluorescein IC3570F
HRP FAB3570H
Janelia Fluor 525 FAB3570JF525
Janelia Fluor 549 FAB3570I
Janelia Fluor 646 FAB3570J
Janelia Fluor 669 FAB3570JF669
PE FAB3570P
PerCP FAB3570C
mFluor Violet 450 SE FAB3570MFV450
mFluor Violet 500 SE FAB3570MFV500
mFluor Violet 610 SE FAB3570MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC3570F
Carrier Free MAB3570-SP
Carrier Free IC3570T-100UG
Carrier Free IC3570S-100UG
Carrier Free IC3570R-100UG
Carrier Free IC3570N-100UG
Carrier Free IC3570G-100UG
Carrier Free IC3570V-100UG
Carrier Free IC3570U-100UG
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344-ER

Recombinant Human EGFR Fc Chimera Protein, CF

(14 Publications)
50 μg
344-ER
Recombinant human EGFR Fc chimera (344-ER) binds recombinant human EGF (236-EG) in a functional ELISA. The estimated Kd for this interaction is < 8 nM.
Hu
Species Glossary
Reactivity: Hu
Binding Activity
Applications: Binding Activity
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DYC1854-2

Human Total EGFR DuoSet IC ELISA, 2 Plate

(9 Publications)
1 Kit (for 2 Plates)
DYC1854-2
ELISA EGFR [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1854E
Biotin DYC1854-5
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MAB1095

EGFR Antibody (102618) [Unconjugated]

(10 Publications)
100 μg
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1095-SP
Carrier Free MAB1095-500
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AF3394

EGFR [p Tyr845] Antibody [Unconjugated]

(4 Publications)
50 μg
AF3394
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y845) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3394), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y845) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, CyTOF-ready, Flow, ICC/IF
Applications: WB, IHC, CyTOF-ready, Flow, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3394-SP
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DEGFR0

Human EGFR Quantikine ELISA Kit

(4 Publications)
1 Kit
DEGFR0
N/A EGFR [HRP]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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MAB89671

EGFR [p Tyr1086] Antibody (938835) [Unconjugated]

(1 Publication)
100 μg
MAB89671
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB89671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB89671-SP
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MAB8967

EGFR [p Tyr1086] Antibody (1240C) [Unconjugated]

(1 Publication)
100 μg
MAB8967
Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8967-SP
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