DDX6 Antibodies
We offer DDX6 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each DDX6 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DDX6 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the DDX6 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DDX6 polyclonal antibodies and browse our DDX6 monoclonal antibody catalog.
Alternate Names for DDX6 Antibodies
anti-DDX6 antibody, anti-ATP-dependent RNA helicase p54 antibody, anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 antibody, anti-DEAD box protein 6 antibody, anti-DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD) antibody, anti-EC 3.6.1 antibody, anti-EC 3.6.4.13 antibody, anti-FLJ36338 antibody, anti-HLR2DEAD box-6 antibody, anti-Oncogene RCK antibody, anti-probable ATP-dependent RNA helicase DDX6 antibody, anti-RCKP54 antibody
11 Results for "ddx6" in Primary Antibodies
![Western Blot: DDX6 Antibody [NB200-191] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NB200-191-img0014.jpg "Western Blot: DDX6 Antibody [NB200-191] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-NB200-191-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NB200-191-img0013.jpg)
![Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunoprecipitation-NB200-191-img0012.jpg)
![Western Blot: DDX6 Antibody [NB200-191] - Staufen1 protein but not mRNA steady-state levels are increased in neurodegenerative disease cells & tissues. Western blot analysis of SCA2- FBs (a) & LBCs (b) show increased STAU1 levels compared with normal controls. DDX6 levels are unchanged. HD & SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal & five SCA2 FBs, & two normal & three SCA2 LBCs were used. c, d Western blot analyses of ATXN2Q127 (c) & BAC-Q72 (d) mouse cerebellar extracts (24 weeks of age) showing increased Stau1 levels compared with wild-type or BAC-Q22 controls (n = 2–3 animals per group). e Western blot of FB extracts from an ALS patient with the TDP-43G298S mutation show increased STAU1 levels. beta -Actin was used as loading control & representative blots of three independent experiments are shown. f–hSTAU1 RNA levels are unaltered in SCA2 & ALS cells & SCA2 mice. qRT-PCR analyses of STAU1 mRNA in SCA2 FBs & ALS FB with TDP-43G298S mutation (f) or SCA2 LBCs (g). h qRT-PCR analyses of cerebellar RNAs from ATXN2Q127 & BAC-Q72 mice compared to wild-type littermates (24 weeks of age; n = animals per group). Gene expression levels were normalized to Actb Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-191_rabbit-polyclonal-ddx6-antibody-310202416212368.jpg)
![Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody NB200-192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NB200-192-img0009.jpg "Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody NB200-192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NB200-192-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX6 Antibody [NB200-192] - Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-192 (lot NB200-192-3) used for IP at 6 ug per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (NB200-192-2) and rabbit anti-DDX6 antibody NB200-191. For blotting immunoprecipitated DDX6, NB200-192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunoprecipitation-NB200-192-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: DDX6 Antibody [NB200-192] - Western blot analysis of HNRNPK and DDX6 protein levels upon HNRNPK or DDX6 knockdown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-12238-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DDX6-Antibody-Knockdown-Validated-NB200-192-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-192] - 5-FU-induced SG assembly is rescued by interfering with RNA incorporation. HeLa cells were treated with 1 mM Urd, 0.1 mM 5-FU or both, as well as with 1 μM FUrd or a combination of 1 μM FUrd & 10 μM Urd. Localization of SG marker protein TIAR (green) & SG/P-body marker protein DDX6 (red) was investigated. Nuclei were stained with Hoechst. Scale bars represent 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24728989), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-192_rabbit-polyclonal-ddx6-antibody-310202415363749.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-192] - 5-FU-induced SG assembly depends on RNA incorporation. (A) Schematic representation of the cellular 5-FU metabolism leading to incorporation of the different metabolites into RNA or DNA. (B) HeLa cells were treated with two concentrations of the 5-FU metabolites FUrd or FdUrd for 72 h. SG marker protein TIAR (green) & SG/P-body marker protein DDX6 (red) were visualized. Nuclei were stained with Hoechst. Scale bars represent 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24728989), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-192_rabbit-polyclonal-ddx6-antibody-310202415371964.jpg)
![Western Blot: DDX6 Antibody [NB200-192] - HNRNPK is necessary for DDX6 to bind differentiation associated mRNAs. a RNA IP was performed in CTLi & HNRNPKi cells using a DDX6 antibody. RT-QPCR was used to determine the levels of binding between DDX6 & differentiation associated mRNAs in the presence or absence of HNRNPK. IGG IPs in CTLi & HNRNPKi cells were used as specificity controls. Binding was calculated as a percent of input. b RNA IP was performed in CTLi & DDX6i cells using a HNRNPK antibody. RT-QPCR was used to determine the levels of binding between HNRNPK & differentiation associated mRNAs in the presence or absence of DDX6. IGG IPs in CTLi & DDX6i cells were used as specificity controls. n = 4. c Western blot analysis of HNRNPK & DDX6 protein levels upon HNRNPK or DDX6 knockdown. d Immunoprecipitations (IPs) were performed using either an HNRNPK or DDX6 antibody or IGG & Western blotted for HNRNPK or DDX6 protein expression. IPs were performed +/− RNase A. Five percent of the cell lysate was used as input. Representative blots are shown. n = 3 independent experiments performed for Fig. 3 unless otherwise indicated. All error bars = SD. ****p < 0.0001, ***p < 0.001 (2 way ANOVA followed by Tukey’s multiple comparison test for a, b) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31519929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-192_rabbit-polyclonal-ddx6-antibody-310202416212349.jpg)
![Western Blot: DDX6 Antibody [NB200-192] - HNRNPK is necessary for DDX6 to bind differentiation associated mRNAs. a RNA IP was performed in CTLi & HNRNPKi cells using a DDX6 antibody. RT-QPCR was used to determine the levels of binding between DDX6 & differentiation associated mRNAs in the presence or absence of HNRNPK. IGG IPs in CTLi & HNRNPKi cells were used as specificity controls. Binding was calculated as a percent of input. b RNA IP was performed in CTLi & DDX6i cells using a HNRNPK antibody. RT-QPCR was used to determine the levels of binding between HNRNPK & differentiation associated mRNAs in the presence or absence of DDX6. IGG IPs in CTLi & DDX6i cells were used as specificity controls. n = 4. c Western blot analysis of HNRNPK & DDX6 protein levels upon HNRNPK or DDX6 knockdown. d Immunoprecipitations (IPs) were performed using either an HNRNPK or DDX6 antibody or IGG & Western blotted for HNRNPK or DDX6 protein expression. IPs were performed +/− RNase A. Five percent of the cell lysate was used as input. Representative blots are shown. n = 3 independent experiments performed for Fig. 3 unless otherwise indicated. All error bars = SD. ****p < 0.0001, ***p < 0.001 (2 way ANOVA followed by Tukey’s multiple comparison test for a, b) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31519929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-192_rabbit-polyclonal-ddx6-antibody-310202416205119.jpg)
![Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DDX6-Antibody-3D2-Western-Blot-H00001656-M01-img0009.jpg "Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DDX6-Antibody-3D2-Immunohistochemistry-Paraffin-H00001656-M01-img0006.jpg)
![Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - Analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # H00001656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/DDX6-Antibody-3D2-Western-Blot-H00001656-M01-img0007.jpg)
![Western Blot: DDX6 Antibody (3D2) [H00001656-M01] - DDX6 monoclonal antibody (M01), clone 3D2 Analysis of DDX6 expression in Hela.](http://images.novusbio.com/fullsize/DDX6-Antibody-3D2-Western-Blot-H00001656-M01-img0008.jpg)
![Western Blot: DDX6 Antibody [NBP1-83249] - Analysis using Anti-DDX6 antibody NBP1-83249 (A) shows similar pattern to independent antibody NBP1-83248 (B).](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NBP1-83249-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX6 Antibody [NBP1-83249] - Analysis using Anti-DDX6 antibody NBP1-83249 (A) shows similar pattern to independent antibody NBP1-83248 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NBP1-83249] - Staining of human cell line A-431 shows localization to cytoplasmic bodies.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83249-img0007.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0013.jpg)
![Western Blot: DDX6 Antibody [NBP1-83249] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NBP1-83249-img0009.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0011.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0012.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0014.jpg)
![Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202414163078.jpg "Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415143678.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP3-35658] - Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-91220241519588.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415121317.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-9122024151959.jpg)
![Western Blot: DDX6 Antibody [NB100-2818] - Staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NB100-2818-img0006.jpg "Western Blot: DDX6 Antibody [NB100-2818] - Staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Thymus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NB100-2818-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NB100-2818-img0007.jpg)
![Western Blot: DDX6 Antibody [H00001656-D01P] - Analysis of DDX6 expression in mouse testis.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-H00001656-D01P-img0007.jpg "Western Blot: DDX6 Antibody [H00001656-D01P] - Analysis of DDX6 expression in mouse testis.")
![Immunocytochemistry/Immunofluorescence: DDX6 Antibody [H00001656-D01P] - Analysis of antibody to DDX6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-H00001656-D01P-img0005.jpg)
![Western Blot: DDX6 Antibody [H00001656-D01P] - Analysis of DDX6 expression in human liver.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-H00001656-D01P-img0006.jpg)
![Western Blot: DDX6 Antibody [H00001656-D01P] - Analysis of DDX6 expression in transfected 293T cell line by DDX6 rabbit polyclonal antibody.Lane 1: DDX6 transfected lysate(53.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-H00001656-D01P-img0008.jpg)
![Western Blot: DDX6 Antibody (6F9I6) [NBP3-33553] - Western blot analysis of various lysates using DDX6 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-33553_rabbit-ddx6-mab-6f9i6-912202414201322.jpg "Western Blot: DDX6 Antibody (6F9I6) [NBP3-33553] - Western blot analysis of various lysates using DDX6 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody (6F9I6) [NBP3-33553] - Immunofluorescence analysis of C6 cells using DDX6 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33553_rabbit-ddx6-mab-6f9i6-91220241581271.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody (6F9I6) [NBP3-33553] - Immunofluorescence analysis of NIH-3T3 cells using DDX6 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33553_rabbit-ddx6-mab-6f9i6-91220241418148.jpg)
![Western Blot: DDX6 Antibody [NBP1-83248] - Analysis using Anti-DDX6 antibody NBP1-83248 (A) shows similar pattern to independent antibody NBP1-83249 (B).](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NBP1-83248-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX6 Antibody [NBP1-83248] - Analysis using Anti-DDX6 antibody NBP1-83248 (A) shows similar pattern to independent antibody NBP1-83249 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83248] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83248-img0006.jpg)
![Western Blot: DDX6 Antibody [NBP1-83248] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-156](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NBP1-83248-img0005.jpg)
![Western Blot: DDX6 Antibody (2D6) [NBP2-46448] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 .](http://images.novusbio.com/fullsize/DDX6-Antibody-2D6-Western-Blot-NBP2-46448-img0002.jpg "Western Blot: DDX6 Antibody (2D6) [NBP2-46448] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX6 .")
![Immunohistochemistry: DDX6 Antibody (2D6) [NBP2-46448] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/DDX6-Antibody-2D6-Immunohistochemistry-NBP2-46448-img0001.jpg)
![Immunohistochemistry: DDX6 Antibody (6D8) [NBP3-26626] - Image of DDX6 Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26626_rabbit-ddx6-mab-6d8-262202414471540.jpg "Immunohistochemistry: DDX6 Antibody (6D8) [NBP3-26626] - Image of DDX6 Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")