DARS Antibodies
We offer DARS Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each DARS Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DARS Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the DARS Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DARS polyclonal antibodies and browse our DARS monoclonal antibody catalog.
Alternate Names for DARS Antibodies
anti-DARS antibody, anti-aspartate tRNA ligase 1, cytoplasmic antibody, anti-Aspartate--tRNA ligase antibody, anti-aspartyl-tRNA synthetase antibody, anti-aspartyl-tRNA synthetase, cytoplasmic antibody, anti-aspRS antibody, anti-Cell proliferation-inducing gene 40 protein antibody, anti-cell proliferation-inducing protein 40 antibody, anti-DKFZp781B11202 antibody, anti-EC 6.1.1.12 antibody, anti-MGC111579 antibody
12 Results for "dars" in Primary Antibodies
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0015.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.")
![Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0011.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0014.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0016.jpg)
![Western Blot: DARS Antibody [NBP1-86027] - Analysis using Anti-DARS antibody NBP1-86027 (A) shows similar pattern to independent antibody NBP1-85938 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-86027-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-86027] - Analysis using Anti-DARS antibody NBP1-86027 (A) shows similar pattern to independent antibody NBP1-85938 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DARS Antibody [NBP1-86027] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86027-img0007.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-86027] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-86027 (A) shows similar protein distribution across tissues to independent antibody NBP1-85938 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-86027-img0008.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human breast shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0006.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human testis.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0010.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human colon.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0011.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human kidney.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebellum, cerebral cortex, colon and kidney using Anti-DARS antibody NBP1-85938 (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebellum, cerebral cortex, colon and kidney using Anti-DARS antibody NBP1-85938 (A) shows similar protein distribution across tissues to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-85938] - Analysis using Anti-DARS antibody NBP1-85938 (A) shows similar pattern to independent antibody NBP1-86027 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85938-img0009.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0015.jpg)
![Western Blot: DARS Antibody [NBP1-85938] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85938-img0007.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0012.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human ceerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0014.jpg)
![Immunohistochemistry: DARS Antibody [NBP3-38289] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DARS Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38289_rabbit-dars-pab-101220241252841.jpg "Immunohistochemistry: DARS Antibody [NBP3-38289] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DARS Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: DARS Antibody [NBP3-38289] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using DARS Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38289_rabbit-dars-pab-101220241243980.jpg)
![Immunoprecipitation: DARS Antibody [NBP3-38289] - Immunoprecipitation analysis of 200 ug extracts of 293T cells using DARS antibody. Western blot was performed from the immunoprecipitate using DARS antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38289_rabbit-dars-pab-1012202411592879.jpg)
![Western Blot: DARS Antibody [NBP3-38289] - Western blot analysis of various lysates using DARS Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38289_rabbit-dars-pab-1012202411552821.jpg)
![Immunohistochemistry: DARS Antibody [NBP3-38289] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using DARS Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38289_rabbit-dars-pab-101220241233872.jpg)
![Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0011.jpg "Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01] - Analysis of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Immunohistochemistry-Paraffin-H00001615-M01-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi ( Cat # H00001615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody (M01), clone 2F11 (Cat # H00001615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0008.jpg)
![Western Blot: DARS Antibody (2F11) [H00001615-M01] - DARS monoclonal antibody (M01), clone 2F11 Analysis of DARS expression in HeLa.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0010.jpg)
![Sandwich ELISA: DARS Antibody (2F11) [H00001615-M01] - Detection limit for recombinant GST tagged DARS is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Sandwich-ELISA-H00001615-M01-img0009.jpg)
![Western Blot: DARS Antibody [NBP1-33689] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-33689-img0005.jpg "Western Blot: DARS Antibody [NBP1-33689] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: DARS Antibody [NBP1-33689] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33689-img0003.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-33689] - SW480 xenograft, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-33689-img0004.jpg)
![Western Blot: DARS Antibody [NBP1-33689] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody [N1C1] (NBP1-33689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33689_rabbit-polyclonal-dars-antibody-910202420104424.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP2-16101] - Human lung cancer, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP2-16101-img0002.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP2-16101] - Human lung cancer, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: DARS Antibody [NBP2-16101] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody (NBP2-16101) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16101_rabbit-polyclonal-dars-antibody-9102024205626.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP3-00268-img0002.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).")
![Immunoprecipitation: DARS Antibody [NBP3-00268] - DARS was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 ul anti-DARS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DARS rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa](http://images.novusbio.com/fullsize/DARS-Antibody-Immunoprecipitation-NBP3-00268-img0003.jpg)
![Immunoprecipitation:DARS AntibodyNBP3-30300] -Detection of human Aspartyl-tRNA Synthetase by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody NBP3-30300 (lot NBP3-30300-1) used for IP at 6 µg per reaction. Aspartyl-tRNA Synthetase was also immunoprecipitated by rabbit anti-Aspartyl-tRNA Synthetase antibody for blotting immunoprecipitated Aspartyl-tRNA Synthetase, NBP3-30300 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30300_rabbit-dars-pab-30122024163367.jpg "Immunoprecipitation:DARS AntibodyNBP3-30300] -Detection of human Aspartyl-tRNA Synthetase by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody NBP3-30300 (lot NBP3-30300-1) used for IP at 6 µg per reaction. Aspartyl-tRNA Synthetase was also immunoprecipitated by rabbit anti-Aspartyl-tRNA Synthetase antibody for blotting immunoprecipitated Aspartyl-tRNA Synthetase, NBP3-30300 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")