CNTFR alpha Antibodies: Novus Biologicals

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4 Results for "cntfr-alpha" in Primary Antibodies

AF-303-NA

CNTFR alpha Antibody [Unconjugated]

(1 Review)

(8 Publications)

100 μg

AF-303-NA

Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recombinant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recombinant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF303

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-303-SP

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AF-559-NA

CNTFR alpha Antibody [Unconjugated]

(3 Publications)

100 μg

AF-559-NA

Recombinant Rat CNTF R alpha (Catalog # 558-CR) enhances Recombinant Rat CNTF (Catalog # 557-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Rat CNTF (10 ng/mL) activity elicited by Recombinant Rat CNTF R alpha (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑559‑NA). The ND50 is typically 2‑6 µg/mL.

CNTF activates tyrosine hydroxylase by phosphorylation in mouse and human locus coeruleusCntfr mRNA expression in mouse locus coeruleus (LC, white arrows). Increasing mRNA content is indicated by a color gradient ranging from dark blue (low) to red (high). The ependymal surface as contact point with the 4th ventricle is shown in blue. Scale bar = 100 μm.Hypothetical orientation of NE neurons with their dendrites emanating toward the 4th ventricle. (B1) CNTF receptor (CNTFR) localization in tyrosine hydroxylase (TH)+ processes extending toward the ventricular wall (arrow marks ventricular surface). Scale bar = 10 μm.c‐Fos activation in LC 2 h after formalin stress. Left: Increased density of c‐Fos+ cells in the LC. Scale bar = 150 μm. Right: Quantitative data. *P 4 mice/genotype.Data information: Data are expressed as means ± s.e.m. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30209240), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Secretagogin expression in rodent brain stem (related to Fig 4)A, A1Labeling specificity of our anti‐secretagogin antibody was verified in Scgn−/− mice (A1). These experiments also showed that tyrosine hydroxylase (TH)+ LC neurons (*) co‐express secretagogin in wild‐type (solid arrowheads) but not null mice (open arrowheads). Scale bars = 200 μm (low‐power micrographs, left) and 10 μm (high‐resolution insets, right).BSecretagogin+ neurons populate the locus coeruleus (LC) in the rat. Solid red circles pinpoint the general location of perikarya, while fine lines correspond to axons coursing toward the surface (4th ventricle).C, C1Secretagogin was expressed in both the somata (arrowheads; C) and dendrites (arrowheads; C1) of most TH+ neurons in the rat, too. Scale bars = 30 μm.DSecretagogin is localized to axons (arrowheads) in the rat. (D) Dorsal nucleus of vagus (nX), “2” shows the nucleus at high resolution. Scale bars = 200 μm (D), 30 μm (2).ESecretagogin (SCGN) is enriched in the synaptosomal fraction prepared from the mouse prefrontal cortex, lending additional support for its axonal localization. Synaptophysin (SYP) was used to control the enrichment of presynaptic proteins.FSecretagogin+ neurons can co‐express neuropeptide Y (NPY) in the LC of the rat. Open and solid arrowheads indicate the lack and existence of NPY co‐localization in adjacent neurons, respectively. Scale bars = 10 μm.GRepresentative Western blots from Cntf−/−, Scgn−/−, and wild‐type mice with or without intracerebroventricular CNTF treatment.H, H1INS‐1E cells were used as a cellular model exclusively because of their native co‐expression of CNTF receptor‐ alpha (CNTFR alpha ), tyrosine hydroxylase (TH), secretagogin (SCGN), and extracellular signal‐regulated kinase 1/2 (Erk1/2). Red arrows label target proteins at their predicted sizes. Multiple bands likely represent splice variants, posttranslational modifications, or multimers. Antibodies were also validated histochemically often using knock‐out tissues.ISecretagogin, phospho‐Ser31‐TH, phospho‐Erk1, and total Erk1 expression in INS‐1E cells stimulated with CNTF with/without concomitant silencing of secretagogin (Scgn) gene expression (for 48 h). Note the lack of Erk1 phosphorylation upon genetic inactivation of secretagogin.JSignificant reduction of secretagogin (SCGN) protein levels upon acute RNAi‐mediated gene silencing in vitro (for 48 h). CNTF exposure did not affect the efficacy of gene silencing. Experiments were performed in triplicate; *P 4 mice/genotype.Data information: Data are expressed as means ± s.e.m. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30209240), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF559

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-559-SP

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MAB303

CNTFR alpha Antibody (121723) [Unconjugated]

(1 Publication)

500 μg

MAB303

CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB303-SP

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BAF559

CNTFR alpha Antibody [Biotin]

50 μg

BAF559

CNTF Ra was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF Ra Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-559-NA
Carrier Free	AF-559-SP

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