ARF1 Antibodies
We offer ARF1 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each ARF1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ARF1 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Zebrafish. Use the list below to choose the ARF1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ARF1 polyclonal antibodies and browse our ARF1 monoclonal antibody catalog.
Alternate Names for ARF1 Antibodies
anti-ARF1 antibody, anti-ADP-ribosylation factor 1 antibody
14 Results for "arf1" in Primary Antibodies
![Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP2-29906-img0012.jpg "Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with an Arf1 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29906-img0007.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of human breast carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0010.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of mouse colon tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0008.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis of showing staining in the cytoplasm of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0009.jpg)
![Immunoprecipitation: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of HeLa whole cell lysate with 2ug of an Arf1 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples, including the HeLa cell lysate as a positive control (left lane), were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a nitrocellulose membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Arf1 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunoprecipitation-NBP2-29906-img0011.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Middle region validated by WB using Fetal lung Lysate at 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0006.jpg "Western Blot: ARF1 Antibody [NBP1-58941] - Middle region validated by WB using Fetal lung Lysate at 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP1-58941] - Mouse brain stem cells, 2 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP1-58941-img0003.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Human Fetal lung Lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0001.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Rat brain homogenate at 1:500.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0002.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Titration: 2 ug/ml Positive Control: Rat brain homogenate](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0005.jpg)
![Western Blot: ARF1 Antibody (1B3) [NBP1-97935] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Western-Blot-NBP1-97935-img0002.jpg "Western Blot: ARF1 Antibody (1B3) [NBP1-97935] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1B3) [NBP1-97935] - Analysis in MCF7 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ARF1 in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Immunocytochemistry-Immunofluorescence-NBP1-97935-img0003.jpg)
![Flow Cytometry: ARF1 Antibody (1B3) [NBP1-97935] - Analysis of ARF1 in MCF7 cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Flow-Cytometry-NBP1-97935-img0005.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0005.jpg "Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).")
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0001.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0002.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0003.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Breast stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0004.jpg)
![Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ARF1-Antibody-0C5B3-Western-Blot-NBP3-16782-img0002.jpg "Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782] - Immunofluorescence analysis of NIH-3T3 cells using ARF1 Rabbit mAb (NBP3-16782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ARF1-Antibody-0C5B3-Immunocytochemistry-Immunofluorescence-NBP3-16782-img0001.jpg)
![Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Western-Blot-NBP3-07760-img0002.jpg "Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).")
![Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) [NBP3-07760] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Immunohistochemistry-Paraffin-NBP3-07760-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody (4G6) [H00000375-M02-100ug] - Antibody to ARF1 on HeLa cell . [antibody concentration 20 ug/ml]](http://images.novusbio.com/fullsize/ARF1-Antibody-4G6-Immunocytochemistry-Immunofluorescence-H00000375-M02-100ug-img0001.jpg "Immunocytochemistry/Immunofluorescence: ARF1 Antibody (4G6) [H00000375-M02-100ug] - Antibody to ARF1 on HeLa cell . [antibody concentration 20 ug/ml]")