ANP32B Antibodies
We offer ANP32B Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each ANP32B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ANP32B Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the ANP32B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ANP32B polyclonal antibodies and browse our ANP32B monoclonal antibody catalog.
Alternate Names for ANP32B Antibodies
anti-ANP32B antibody, anti-acidic (leucine-rich) nuclear phosphoprotein 32 family, member B antibody, anti-acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, anti-Acidic protein rich in leucines antibody, anti-APRILSilver-stainable protein SSP29 antibody, anti-PHAPI2SSP29 antibody, anti-Putative HLA-DR-associated protein I-2 antibody
8 Results for "anp32b" in Primary Antibodies
![Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0002.jpg "Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NB100-1199] - LPS/IFN gamma-stimulated neuron-glial co-cultures (Glia/PAL31+ neuron) showing expression of both iNOS and PAL31. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1199-img0006.jpg)
![Immunohistochemistry: ANP32B Antibody [NB100-1199] - (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) and 1000X (E&F). Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-NB100-1199-img0005.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0001.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody [NB100-1199] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-Paraffin-NB100-1199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: ANP32B Antibody [NB100-1199] - Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and 166 (lane 3) picomole of pal31 siRNA or 332 picomole negative control. Actin works as a loading control. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Knockdown-Validated-NB100-1199-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Upregulated PAL31 expression was observed not only in microglia/ macrophage but also in glial cells near the injury site. (A) Normal thoracic spinal cord section showing few ED-1 positive cells (red). (B) Normal thoracic spinal cord section showing GFAP positive cells (red). (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) & 1000X (E&F). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-3102024168397.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Overexpression of PAL31 in C6 did not affect the cell proliferative activity. (A) GFP/bright field-superimposed micrograph in GFP overexpressing cells showing > 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-310202415525215.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - Effect of overexpression or knockdown of PAL31 in C6 on H2O2-induced toxicity. (A) Representative micrographs showing C6 overexpressing GFP or GFP-tagged PAL31 after being treated with H2O2 (0 ~ 1 mM) for 4 hours. (B) MTT assay in GFP- or GFP-PAL31-overexpressed C6 after H2O2 treatment showing significant difference (cytoprotective effect of PAL31) at 0.5 mM & 1 mM H2O2 treatment between C6/GFP & C6/PAL31 groups. The data in each dosage were analyzed by two-way ANOVA & Bonferroni post hoc test. *P 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-31020241682198.jpg)
![Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199] - Western blot analysis of extracts of various cell lines, using ANP32B Rabbit mAb (NBP3-16199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Western-Blot-NBP3-16199-img0006.jpg "Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199] - Western blot analysis of extracts of various cell lines, using ANP32B Rabbit mAb (NBP3-16199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunofluorescence analysis of NIH-3T3 cells using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Immunocytochemistry-Immunofluorescence-NBP3-16199-img0005.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry of paraffin-embedded mouse kidney using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Immunohistochemistry-Paraffin-NBP3-16199-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunofluorescence analysis of C6 cells using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Immunocytochemistry-Immunofluorescence-NBP3-16199-img0004.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry of paraffin-embedded rat spleen using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Immunohistochemistry-Paraffin-NBP3-16199-img0001.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry of paraffin-embedded human thyroid cancer using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Immunohistochemistry-Paraffin-NBP3-16199-img0002.jpg)
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Western Blot: ANP32B Antibody (SR1488) [NBP3-22127] - Western blot analysis using NBP3-22127 in (1) Jurkat cell lysate; (2) RAW264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22127_rabbit-anp32b-mab-sr1488-57202316582132.jpg "Western Blot: ANP32B Antibody (SR1488) [NBP3-22127] - Western blot analysis using NBP3-22127 in (1) Jurkat cell lysate; (2) RAW264.7 cell lysate.")
![Western Blot: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10. Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ANP32B-Antibody-8E10-Western-Blot-H00010541-M05-img0004.jpg "Western Blot: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10. Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of monoclonal antibody to ANP32B on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ANP32B-Antibody-8E10-Immunocytochemistry-Immunofluorescence-H00010541-M05-img0002.jpg)
![ELISA: ANP32B Antibody (8E10) [H00010541-M05] - Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ANP32B-Antibody-8E10-Sandwich-ELISA-H00010541-M05-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4F5) [NBP3-42928] - Immunofluorescent analysis of HepG2 cells using (ANP32B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)](http://images.novusbio.com/fullsize/nbp3-42928_mouse-anp32b-mab-4f5-101220241715441.jpg "Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4F5) [NBP3-42928] - Immunofluorescent analysis of HepG2 cells using (ANP32B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)")
![Western Blot: ANP32B Antibody (4F5) [NBP3-42928] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (ANP32B Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-42928_mouse-anp32b-mab-4f5-101220241723672.jpg)
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NBP3-17193] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17193-img0001.jpg "Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NBP3-17193] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Western Blot: ANP32B Antibody [NBP2-88766] - Host: Rabbit. Target Name: AN32B. Sample Type: MCF7 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NBP2-88766-img0001.jpg "Western Blot: ANP32B Antibody [NBP2-88766] - Host: Rabbit. Target Name: AN32B. Sample Type: MCF7 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")