Alpha-mannosidase II Antibodies
We offer Alpha-mannosidase II Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each Alpha-mannosidase II Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Alpha-mannosidase II Antibodies can be used in a variety of model species: Human. Use the list below to choose the Alpha-mannosidase II Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Alpha-mannosidase II polyclonal antibodies and browse our Alpha-mannosidase II monoclonal antibody catalog.
Alternate Names for Alpha-mannosidase II Antibodies
anti-Alpha-mannosidase II antibody, anti-MAN2A1 antibody, anti-alpha-mannosidase 2 antibody, anti-AMan II antibody, anti-EC 3.2.1 antibody, anti-EC 3.2.1.114 antibody, anti-Golgi alpha-mannosidase II antibody, anti-golgi integral membrane protein 7 antibody, anti-GOLIM7 antibody, anti-Man II antibody, anti-MANA2MANII antibody, anti-Mannosidase alpha class 2A member 1 antibody, anti-mannosidase, alpha type II antibody, anti-mannosidase, alpha, class 2A, member 1 antibody, anti-Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase antibody
4 Results for "alpha-mannosidase-ii" in Primary Antibodies
![Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-27820241631657.jpg "Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29820241420970.jpg)
![Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Flow cytometric analysis of U-87 MG cells labeling Alpha-mannosidase II. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32565, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29202416372118.jpg)
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0007.jpg "Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0005.jpg)
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining in human parathyroid gland and skeletal muscle tissues using anti-MAN2A1 antibody. Corresponding MAN2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0008.jpg)
![Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Western-Blot-NB100-93414-img0005.jpg "Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).")
![Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - Analysis of alpha-mannosidase II antibody [NB100-93414] (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Western-Blot-NB100-93414-img0004.jpg)
![Western Blot: Alpha-mannosidase II Antibody (1G9) [H00004124-M01] - Detection against Immunogen (36.74 KDa) .](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-1G9-Western-Blot-H00004124-M01-img0001.jpg "Western Blot: Alpha-mannosidase II Antibody (1G9) [H00004124-M01] - Detection against Immunogen (36.74 KDa) .")