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Semicircular Canal Formation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Semicircular Canal Formation Pathway and Circling Gait, Tissue Adhesions, Crest Syndrome, Nervousness, Cell Transformation, Neoplastic. The study of the Semicircular Canal Formation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Semicircular Canal Formation Pathway has been researched in relation to Ear Development, Inner Ear Development, Inner Ear Morphogenesis, Ear Morphogenesis, Cell Proliferation. The Semicircular Canal Formation Pathway complements our catalog of research reagents including antibodies and ELISA kits against NEURONAL CALCIUM SENSOR-1, NCS-1, BMP2, BMP4, CDH2.

Top Research Reagents

We have 2943 products for the study of the Semicircular Canal Formation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Otx1 Antibody (PSH01-62)
NBP3-32682
Western Blot: Otx1 Antibody (PSH01-62) [NBP3-32682] - Western blot analysis of Otx1 on different lysates with Rabbit anti-Otx1 antibody (NBP3-32682) at 1/1,000 dilution.<br/><br/> Lane 1: 293T transfected with FLAG-tagged empty control cell lysate<br/> Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 32 kDa<br/> Observed band size: 32 kDa<br/><br/> Exposure time: 24 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32682) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.Immunohistochemistry: Otx1 Antibody (PSH01-62) [NBP3-32682] - Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-Otx1 antibody (NBP3-32682) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32682) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

PELP1 Antibody
NB200-331
Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

18 Publications
N-Cadherin Antibody (13A9)
NBP1-48309
Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (https://www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     2 Reviews

41 Publications
HMX3 Antibody
NBP1-69011
Western Blot: HMX3 Antibody [NBP1-69011] - analysis of HMX3 in HeLa cell lysate using anti-HMX3 antibody. Image from verified customer review.Western Blot: HMX3 Antibody [NBP1-69011] - Mouse Liver lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB

     1 Review

TMIE Antibody
NBP1-84260
Immunohistochemistry-Paraffin: TMIE Antibody [NBP1-84260] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

RNF7 Antibody
NBP1-85594
Western Blot: RNF7 Antibody [NBP1-85594] - Analysis in control (vector only transfected HEK293T lysate) and RNF7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: RNF7 Antibody [NBP1-85594] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

Glutathione S Transferase kappa 1 Antibody
NBP2-16686
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Wild-type (WT) and Glutathione S Transferase kappa 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S Transferase kappa 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
LRIG1 Antibody [Unconjugated]
AF3688
Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, Flow, CyTOF-ready

35 Publications
SOX2 Antibody [Unconjugated]
AF2018
Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (<a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     6 Reviews

364 Publications
IGFBP-3 [HRP]
DGB300
N/A IGFBP-3 [HRP]N/A IGFBP-3 [HRP]


Species Human
Applications ELISA

47 Publications
BMP-2 [Unconjugated]
355-BM
Recombinant human/mouse/rat BMP-2 (<a class=NoLineLink href=


Species Human, Mouse, Rat
Applications BA

189 Publications
ErbB2/Her2 [Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.Recombinant human ErB2 / Her2 (<a class=


Species Human
Applications BA

58 Publications
BMP-4 [Unconjugated]
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

512 Publications
Netrin-1 [Unconjugated]
1109-N1
<P align=left>1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.


Species Mouse
Applications Bind

40 Publications
ErbB3/Her3 Antibody (66223) [Unconjugated]
MAB3481
Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications Flow, CyTOF-ready, ELISA(Cap)

21 Publications
MIF Antibody (12302) [Unconjugated]
MAB289
Western blot shows lysate of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class= Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MIF at approximately 12 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) . This experiment was conducted under reducing conditions and using the <br>12-230 kDa separation system.        

Mouse Monoclonal
Species Human
Applications WB, Simple Western

     1 Review

28 Publications
LMO4 Antibody
NBP2-49342
Immunocytochemistry/Immunofluorescence: LMO4 Antibody [NBP2-49342] - Staining of human cell line SiHa shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: LMO4 Antibody [NBP2-49342] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

Smad5 [p Ser463, p Ser465] Antibody (SY09-03)
NBP2-67394
Western Blot: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brainImmunocytochemistry/Immunofluorescence: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication