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![Western Blot: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Western-Blot-NBP2-67394-img0008.jpg "Western Blot: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain")
![Immunocytochemistry/Immunofluorescence: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunocytochemistry-Immunofluorescence-NBP2-67394-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Staining Phospho-Smad5(S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunocytochemistry-Immunofluorescence-NBP2-67394-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad5 [p Ser463, p Ser465] Antibody (SY09-03) [NBP2-67394] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5(S463/465) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad5-p-Ser463-p-Ser465-Antibody-SY09-03-Immunohistochemistry-Paraffin-NBP2-67394-img0006.jpg)
![Western Blot: Smad5 Antibody (3H9) [NBP2-37648] - Analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.](http://images.novusbio.com/fullsize/Smad5-Antibody-3H9-Western-Blot-NBP2-37648-img0001.jpg "Western Blot: Smad5 Antibody (3H9) [NBP2-37648] - Analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad5 Antibody (3H9) [NBP2-37648] - Analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad5-Antibody-3H9-Immunocytochemistry-Immunofluorescence-NBP2-37648-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad5 Antibody (3H9) [NBP2-37648] - Analysis of tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad5-Antibody-3H9-Immunohistochemistry-Paraffin-NBP2-37648-img0005.jpg)
![Flow Cytometry: Smad5 Antibody (3H9) [NBP2-37648] - Analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Smad5-Antibody-3H9-Flow-Cytometry-NBP2-37648-img0003.jpg)
![Western Blot: SMAD5 Antibody [NBP2-20413] - SMAD5 antibody detects SMAD5 protein by Western blot analysis. A. 30 ug Raw264. 7 whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract10 % SDS-PAGESMAD5 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/SMAD5-Antibody-Western-Blot-NBP2-20413-img0003.jpg "Western Blot: SMAD5 Antibody [NBP2-20413] - SMAD5 antibody detects SMAD5 protein by Western blot analysis. A. 30 ug Raw264. 7 whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract10 % SDS-PAGESMAD5 antibody dilution: 1:1000.")
![Western Blot: SMAD5 Antibody [NBP2-20413] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMAD5-Antibody-Western-Blot-NBP2-20413-img0001.jpg)
![Western Blot: SMAD5 Antibody [NBP2-20413] - SMAD5 antibody detects SMAD5 protein by Western blot analysis. A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGESMAD5 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/SMAD5-Antibody-Western-Blot-NBP2-20413-img0002.jpg)
![Western Blot: Smad5 Antibody [NBP2-20413] - Smad5 antibody detects smad5 protein by western blot analysis. Various zebrafish tissue extracts (30 ug) were separated by 10 % SDS-PAGE, and the membrane was blotted with Smad5 antibody (NBP2-20413) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp2-20413_rabbit-polyclonal-smad5-antibody-91020242001096.jpg)
![Immunohistochemistry: Smad5 Antibody [NBP2-20413] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Smad5 antibody (NBP2-20413) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-20413_rabbit-polyclonal-smad5-antibody-910202420134063.jpg)
![Western Blot: Smad5 Antibody (2D7) [H00004090-M01] - SMAD5 monoclonal antibody (M01), clone 2D7. Analysis of SMAD5 expression in K-562.](http://images.novusbio.com/fullsize/Smad5-Antibody-2D7-Western-Blot-H00004090-M01-img0016.jpg "Western Blot: Smad5 Antibody (2D7) [H00004090-M01] - SMAD5 monoclonal antibody (M01), clone 2D7. Analysis of SMAD5 expression in K-562.")
![Immunocytochemistry/Immunofluorescence: Smad5 Antibody (2D7) [H00004090-M01] - Analysis of monoclonal antibody to SMAD5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Smad5-Antibody-2D7-Immunocytochemistry-Immunofluorescence-H00004090-M01-img0012.jpg)
![Western Blot: Smad5 Antibody (2D7) [H00004090-M01] - SMAD5 monoclonal antibody (M01), clone 2D7 Analysis of SMAD5 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Smad5-Antibody-2D7-Western-Blot-H00004090-M01-img0014.jpg)
![Western Blot: Smad5 Antibody (2D7) [H00004090-M01] - SMAD5 monoclonal antibody (M01), clone 2D7. Analysis of SMAD5 expression in Jurkat.](http://images.novusbio.com/fullsize/Smad5-Antibody-2D7-Western-Blot-H00004090-M01-img0015.jpg)
![Sandwich ELISA: Smad5 Antibody (2D7) [H00004090-M01] - Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad5-Antibody-2D7-Sandwich-ELISA-H00004090-M01-img0013.jpg)
![Western Blot: Smad5 Antibody [NBP2-32406] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Smad5-Antibody-Western-Blot-NBP2-32406-img0009.jpg "Western Blot: Smad5 Antibody [NBP2-32406] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: Smad5 Antibody [NBP2-32406] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Smad5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32406-img0014.jpg)
![Immunohistochemistry-Paraffin: Smad5 Antibody [NBP2-32406] - Staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad5-Antibody-Immunohistochemistry-Paraffin-NBP2-32406-img0015.jpg)
![Western Blot: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] - Western blot analysis using NBP3-21515 in (1) Mouse brain tissue lysate; (2) Rat brain tissue lysate.](http://images.novusbio.com/fullsize/nbp3-21515_rabbit-smad5-p-ser-463-mab-sr2030-5720231521381.jpg "Western Blot: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] - Western blot analysis using NBP3-21515 in (1) Mouse brain tissue lysate; (2) Rat brain tissue lysate.")
![Immunohistochemistry: Smad5 [p Ser 463] Antibody (SR2030) [NBP3-21515] - Immunohistochemical analysis of paraffin-embedded human pancreas, using NBP3-21515.](http://images.novusbio.com/fullsize/nbp3-21515_rabbit-smad5-p-ser-463-mab-sr2030-57202316112129.jpg)
![Western Blot: Smad5 Antibody (3A10) [H00004090-M03] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad5-Antibody-3A10-Western-Blot-H00004090-M03-img0009.jpg "Western Blot: Smad5 Antibody (3A10) [H00004090-M03] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).")
![Immunocytochemistry/Immunofluorescence: Smad5 Antibody (3A10) [H00004090-M03] - Analysis of monoclonal antibody to SMAD5 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Smad5-Antibody-3A10-Immunocytochemistry-Immunofluorescence-H00004090-M03-img0008.jpg)
![ELISA: Smad5 Antibody (3A10) [H00004090-M03] - Detection limit for recombinant GST tagged SMAD5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad5-Antibody-3A10-ELISA-H00004090-M03-img0004.jpg)
![Immunoprecipitation: Smad5 Antibody (1X5G9) [NBP3-33193] - Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3 ug Smad5 antibody. Western blot was performed from the immunoprecipitate using Smad5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-33193_rabbit-smad5-mab-1x5g9-1012202411343323.jpg "Immunoprecipitation: Smad5 Antibody (1X5G9) [NBP3-33193] - Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3 ug Smad5 antibody. Western blot was performed from the immunoprecipitate using Smad5 antibody at a dilution of 1:1000.")
![Western Blot: Smad5 Antibody (1X5G9) [NBP3-33193] - Western blot analysis of lysates from K-562 cells, using Smad5 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-33193_rabbit-smad5-mab-1x5g9-1012202411343391.jpg)
![Western Blot: Smad5 Antibody (1X5G9) [NBP3-33193] - Western blot analysis of lysates from C2C12 cells, using Smad5 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33193_rabbit-smad5-mab-1x5g9-1012202411353087.jpg)
![Western Blot: Smad5 [p Ser 463] Antibody (SR1930) [NBP3-21556] - Western blot analysis using NBP3-21556 in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate.](http://images.novusbio.com/fullsize/nbp3-21556_rabbit-smad5-p-ser-463-mab-sr1930-5720231754847.jpg "Western Blot: Smad5 [p Ser 463] Antibody (SR1930) [NBP3-21556] - Western blot analysis using NBP3-21556 in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate.")
![Western Blot: Smad5 Antibody (4F10) [H00004090-M05] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad5-Antibody-4F10-Western-Blot-H00004090-M05-img0004.jpg "Western Blot: Smad5 Antibody (4F10) [H00004090-M05] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).")
![Sandwich ELISA: Smad5 Antibody (4F10) [H00004090-M05] - Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad5-Antibody-4F10-Sandwich-ELISA-H00004090-M05-img0003.jpg)
![Western Blot: Smad5 Antibody (5E12) [H00004090-M08] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad5-Antibody-5E12-Western-Blot-H00004090-M08-img0003.jpg "Western Blot: Smad5 Antibody (5E12) [H00004090-M08] - Analysis of SMAD5 expression in HeLa (Cat # L013V1).")
![ELISA: Smad5 Antibody (5E12) [H00004090-M08] - Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad5-Antibody-5E12-ELISA-H00004090-M08-img0002.jpg)