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Protein Acylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Protein Acylation Pathway and Diabetes Mellitus, Diabetes Mellitus, Non-insulin-dependent, Insulin Resistance, Obesity, Metabolic Diseases. The study of the Protein Acylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Protein Acylation Pathway has been researched in relation to Secretion, Insulin Secretion, Localization, Transport, Exocytosis. The Protein Acylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against INS, TNF, PRRT2, NMT1, INSR.

Top Research Reagents

We have 3740 products for the study of the Protein Acylation Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP1-86013
Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Analysis in control (vector only transfected HEK293T lysate) and SIRT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415885).Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human Stomach shows moderate granular cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP1-89522
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Analysis in human kidney and pancreas tissues. Corresponding DBT RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-89522 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-90276
Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276] - Analysis in human cell line RPMI-8226.Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

2 Publications
NBP1-92440
Immunohistochemistry-Paraffin: SPNS1 Antibody [NBP1-92440] - Staining in human lung and pancreas tissues using NBP1-92440 antibody. Corresponding SPNS1 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: SPNS1 Antibody [NBP1-92440] - Staining of human lung, pancreas, placenta and testis using Anti-SPNS1 antibody NBP1-92440 (A) shows similar protein distribution across tissues to independent antibody NBP1-92441 (B).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP2-01743
Western Blot: Frataxin Antibody (1C12) [NBP2-01743] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Frataxin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Frataxin.Immunocytochemistry/Immunofluorescence: Frataxin Antibody (1C12) [NBP2-01743] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Frataxin.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

NBP2-12793
Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review. Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples:  Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

3 Publications
NBP2-27104
Western Blot: LAT Antibody [NBP2-27104] - analysis of LAT in mouse thymus tissue lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: LAT Antibody [NBP2-27104] - FFPE human small intestine stained with LAT antibody at 10 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Primate
Applications WB, IHC, IHC-P

NB100-1533
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

10 Publications
MAB1417
Insulin was detected in immersion fixed  beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

24 Publications
MAB5285
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAG1 Polyclonal Antibody (Catalog # MAB5285) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PAG1 at approximately 76 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PAG1 Monoclonal Antibody (Catalog # MAB5285). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. </P>

Mouse Monoclonal
Species Human
Applications WB, Simple Western

1 Publication
NBP2-37399
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.Immunocytochemistry/Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

NB100-2564
Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.

Rabbit Polyclonal
Species Human
Applications WB

1 Publication
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

807 Publications
NBP2-42864
Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NBP2-44501
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications Flow, ICC/IF, IHC

     1 Review

NBP2-52563
Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Cochlear whole mount from wild-type and Sirt3-KO mice, immunolabeled with MYO7, SIRT3, and DAPI. The cross-hairs show the position from which the side view is taken. SIRT3 immunoreactivity only is shown in the right panels. IHC-WhMt image submitted by a verified customer review.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

     1 Review

NBP2-82026
Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NBP2-92856
Western Blot: ACBP Antibody [NBP2-92856] - Analysis of extracts of various cell lines, using DBI antibody  at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.Immunocytochemistry/Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of U2OS cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP3-12294
Western Blot: Chimaerin 2 Antibody [NBP3-12294] - 1:1000 antibody dilution in DiluObuffer. Apparent MW is 50 KDa.Immunohistochemistry-Paraffin: Chimaerin 2 Antibody [NBP3-12294] - Rat cerebellum. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

NBP3-12963
Western Blot: NMT1 Antibody [NBP3-12963] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (NBP3-12963) diluted at 1:5000.Immunocytochemistry/Immunofluorescence: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMT1 protein stained by NMT1 antibody (NBP3-12963) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC