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Primary Sex Determination Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Primary Sex Determination Pathway and Disorders Of Sex Development, Hermaphroditism, Sex Chromosome Aberrations, Xx Male, True Hermaphroditism (disorder). The study of the Primary Sex Determination Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Primary Sex Determination Pathway has been researched in relation to Sex Determination, Dosage Compensation, Spermatogenesis, Cell Differentiation, Meiosis. The Primary Sex Determination Pathway complements our catalog of research reagents including antibodies and ELISA kits against TRA-1, FKH-6, T-ASSOCIATED SEX REVERSAL, TAS, STS.

Top Research Reagents

We have 1248 products for the study of the Primary Sex Determination Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Xanthine Oxidase Antibody (0M8E7)
NBP3-16735
Western Blot: Xanthine Oxidase Antibody (0M8E7) [NBP3-16735] - Western blot analysis of extracts of various cell lines, using Xanthine Oxidase (XDH) Rabbit mAb (NBP3-16735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Immunocytochemistry/ Immunofluorescence: Xanthine Oxidase Antibody (0M8E7) [NBP3-16735] - Confocal imaging of paraffin-embedded Human liver using Xanthine Oxidase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

HMGB2 Antibody (8P10V5)
NBP3-16774
Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of 300ug extracts of Mouse testis cells using 3ug HMGB2 antibody (NBP3-16774). Western blot was performed from the immunoprecipitate using HMGB2 (NBP3-16774) at a dilition of 1:1000.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Aromatase Antibody
NB100-1596
Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

16 Publications
ZFX Antibody
NBP1-80582
Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.Immunohistochemistry-Paraffin: ZFX Antibody [NBP1-80582] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

4 Publications
DMRT1 Antibody
NBP1-84071
Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Staining in human testis and fallopian tube tissues using anti-DMRT1 antibody. Corresponding DMRT1 RNA-seq data are presented for the same tissues.Western Blot: DMRT1 Antibody [NBP1-84071] - Analysis in control (vector only transfected HEK293T lysate) and DMRT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

4 Publications
Steroid sulfatase Antibody
NBP1-90095
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human placenta shows high expression.Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human pancreas shows low expression as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

3 Publications
UBL4A Antibody (OTI1D4)
NBP2-03621
Western Blot: UBL4A Antibody (1D4) [NBP2-03621] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBL4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBL4A.Immunocytochemistry/Immunofluorescence: UBL4A Antibody (1D4) [NBP2-03621] Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBL4A.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

A2BP1 Antibody (D8H8)
NBP2-13169
Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Mouse Monoclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

4 Publications
HMGN1/HMG14 Antibody
NBP2-14842
Western Blot: HMG14 Antibody [NBP2-14842] - Analysis of HMG14 in Daudi lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of HMG14 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Rabbit Polyclonal
Species Human, Porcine, Bovine
Applications WB

Recombinant Human HMGN2 GST (N-Term) Protein
H00003151-P01
SDS-Page: HMGN2 Recombinant Protein [H00003151-P01]


Species Human
Applications WB, ELISA, PA

1 Publication
SOX9 Antibody [Unconjugated]
AF3075
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, ICC

     2 Reviews

155 Publications
GLI-2 Antibody
AF3635
GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC

     2 Reviews

44 Publications
Elastase Antibody (CELA3B/1257) - Azide and BSA Free
NBP2-54301
Western Blot: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Western Blot Analysis of PANC-1 cell lysate using Elastase antibody (CELA3B/1257).Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

HMGB1/HMG-1 Antibody
NB100-2322
Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Rabbit Anti-Human HMGB1 Polyclonal Antibody (Catalog # NB100-2322) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HMGB1 at approximately 30 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions. Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HMGB1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     5 Reviews

40 Publications